干旱区生态环境知识资源中心(Arid): 饮料导致釉质组织和形态学改变的观察

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	饮料导致釉质组织和形态学改变的观察
其他题名	Observation of histological and morphological alteration on bovine enamel caused by several beverages
	刘兴容 1; 靳淑风 2; 周学东 3; 张萍 3
来源期刊	牙体牙髓牙周病学杂志
ISSN	1005-2593
出版年	2003
卷号	13 期号:1 页码:7-10,T001
中文摘要	目的：观察人牙本质涎蛋白对体外培养的人牙乳头间充质细胞增殖和碱性磷酸酶活性的影响。方法：分对照组和实验组，对照组是转染不含目的基因的空白质粒pcDNA3的培养上清，实验组是转染pcDNA3-hDSP重组质粒的培养上清。取第5代人牙乳头间充质细胞，接种于96孔板，对照组6孔，实验组10孔，用MTT法检测hDSP对体外培养的人牙乳头间充质细胞增殖的影响，用碱性磷酸酶检测试剂盒检测hDSP对体外培养的人牙乳头间充质细胞碱性磷酸酶活性的影响。结果：hDSP能明显抑制体外培养的人牙乳头间充质细胞增殖（P<0.01），但能促进细胞内和培养上清中碱性磷酸酶的分泌（P<0.01）。结论：hDSP能促进人牙乳头间充质细胞的分化和矿化。目的：观察不同种类饮料对釉质组织和形态学的改变。方法：采用体视显微镜、偏光显微镜和扫描电镜观察不同种类饮料浸泡釉质后，釉质表面超微结构和组织学的改变，饮料的处理是37℃，每天10次，每次5min。设去离子水为对照组。结果：体视显微镜观察表明，除矿泉水外，其余饮料导致釉质表面不同程度组织缺失，有色饮料能导致釉质表面颜色的改变且随处理时间的延长而加深。偏光显微镜观察表明磨片浸喹啉后脱矿区呈负性双折射，而浸水时则呈正性双折射。扫描电镜也观察到釉柱出现不同程度的脱矿，可乐和橙汁的脱矿作用较钙奶强。矿泉水和去离子水处理的釉质未见脱矿征象。结论：实验饮料除矿泉水外均能导致釉质脱矿。
英文摘要	AIM;To observe ihe hislological and morphological alleralion on bovine enamel surface causedby several kinds of beverages. METHODS: All ihe beverages were purchased from local supermarket randomly. After 7 days’exposure to the beverages, 5 ruin each lime and 10 times each day, ihe hislological and morphological alteration of bovine enamels were analy/ed by slereomicroscopy, polarized lighl microscopy arid scanning electron microscope. RESULTS :Slereomicroscopy showed all the specimens except those treated by mineral water lost iheir gloss and had irregular loss of dental tissue in variable degrees. It also revealed thai colored beverages could cause the alteration of normal enamel color. The longer the enamel expose to the beverage, ihe deeper the color of the enamels was. Polarized light microscope showed that the demineralized area revealed negative birefringence when the ground sections were examined in quinoline and positive birefringence when the ground sections were examined in water. Different degrees of solubili-zation of enamel prisms were demonstrated by scanning electron microscope. The demineralizalion caused by Future - cola and orange juice was more severe than that caused by Robust Calcium -milk. CONCLUSION; All the beverages except mineral water have erosive effect on bovine enamel, but among ihem ihe calcium -milk is lowest.
中文关键词	饮料 ; 釉质 ; 体视显微镜 ; 偏光显微镜 ; 扫描电镜
英文关键词	beverages enamel stereomicroscopy polarized light microscopy scanning electron microscope
语种	中文
国家	中国
收录类别	CSCD
WOS类目	DENTISTRY ORAL SURGERY MEDICINE
WOS研究方向	Dentistry, Oral Surgery & Medicine
CSCD记录号	CSCD:1286936
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/204509
作者单位	1.泸州医学院附属医院, 泸州, 四川 646000, 中国; 2.天津市口腔医院, 天津 300000, 中国; 3.四川大学华西口腔医院, 成都, 四川 610041, 中国
推荐引用方式 GB/T 7714	刘兴容,靳淑风,周学东,等. 饮料导致釉质组织和形态学改变的观察[J],2003,13(1):7-10,T001.
APA	刘兴容,靳淑风,周学东,&张萍.(2003).饮料导致釉质组织和形态学改变的观察.牙体牙髓牙周病学杂志,13(1),7-10,T001.
MLA	刘兴容,et al."饮料导致釉质组织和形态学改变的观察".牙体牙髓牙周病学杂志 13.1(2003):7-10,T001.