干旱区生态环境知识资源中心(Arid): 反相高效液相色谱法测定全血中马钱子碱和士的宁的含量

Arid

	反相高效液相色谱法测定全血中马钱子碱和士的宁的含量
其他题名	Determination of Brucine and Strychnine in Human Blood by Reversed-Phase High Performance Liquid Chromatography
	王朝虹 1; 果德安 1; 胡春华 2
来源期刊	色谱
ISSN	1000-8713
出版年	2003
卷号	21 期号:4 页码:382-384
中文摘要	建立了反相高效液相色谱法定量分析全血中马钱子碱和士的宁含量的方法。该方法应用Oasis MCX固相萃取小柱进行提取、采用XTerra~(TM)RP_(18)色谱柱进行分离。在该条件下马钱子碱和士的宁的线性范围为0.05～10 mg/L，最小检出限为0.15 ng。该方法测得马钱子碱和士的宁在低、中、高浓度下的回收率均在90%以上，日内、日间精密度均符合方法学要求。该方法灵敏、快速、准确，适用于临床血药浓度监测和法医毒化检验。
英文摘要	A high performance liquid chromatographic (HPLC) method was developed for the simulta-neous determination of brucine and strychnine in human blood. The samples were pretreated with solid phase extraction using Oasis~? MCX cartridges and the alkaloids were separated on Xterra~1 RP_(18) column with UV detection at 254 nm. Calibration curves were linear within the range of 0.05 - 10 mg/L and the limit of detection was 0.15 ng for each alkaloid. The recoveries obtained from spiked blood for the two alkaloids were more than 90 %. The intra-day and inter-day presisions were assessed at three mass concentration levels (0.5,1.0,2.0 mg/L), and the relative standard deviations were in the range of 1.7% to 9.4% . The method described was proved to be sensitive, rapid and accurate, and it can be applied to the identification and determination of the alkaloids for forensic and therapeutic drugs moni-toring.
中文关键词	固相萃取 ; 高效液相色谱法 ; 马钱子碱 ; 士的宁
英文关键词	solid-phase extraction high performance liquid chromatography brucine strychnine
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:1175086
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/204254
作者单位	1.北京大学药学院, 北京 100083, 中国; 2.公安部物证鉴定中心, 北京 100038, 中国
推荐引用方式 GB/T 7714	王朝虹,果德安,胡春华. 反相高效液相色谱法测定全血中马钱子碱和士的宁的含量[J],2003,21(4):382-384.
APA	王朝虹,果德安,&胡春华.(2003).反相高效液相色谱法测定全血中马钱子碱和士的宁的含量.色谱,21(4),382-384.
MLA	王朝虹,et al."反相高效液相色谱法测定全血中马钱子碱和士的宁的含量".色谱 21.4(2003):382-384.