干旱区生态环境知识资源中心(Arid): PCR-SSCP检测急性白血病患者N-ras基因突变及临床意义初探

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	PCR-SSCP检测急性白血病患者N-ras基因突变及临床意义初探
	刘红 1; 彭家和 2; 钟小林 2; 曹小林 2; 张立 2; 何凤田 2
来源期刊	第三军医大学学报
ISSN	1000-5404
出版年	2003
卷号	25 期号:7 页码:629-631
中文摘要	目的:探讨N-ras基因突变与急性白血病发生的关系。方法:采取骨髓和外周血白细胞提取DNA,用PCR-SSCP分析技术分别对急性白血病患者、正常对照组的N-ras基因突变进行检测。结果:84例急性白血病患者中25例发生N-ras基因突变,基因变异频率为29.8%,其中急性淋巴细胞白血病为18.6%,急性髓细胞白血病为41.4%;11例随访6个月,7例N-ras基因突变消失,4例N-ras基因突变持续存在;正常对照组未发现N-ras基因突变。结论:N-ras基因突变对急性白血病患者的预后具有一定的参考价值。
英文摘要	Objective To explore the relationship between N-ms mutation and the pathogenesis of acute leuke-mia. Msthods Human genomie DNA was extracted from normal human peripheral white blood cell and bone marrow of patients. N-ras gene mutations in 84 cases of acute leukemia and 50 normal healthy cases were detected by PCR-SSCP technique, respectively. Results N-ras gene mutations were found in 25 eases (tut of 84 cases of acute leuke-mia with the frequency of N-ras gene variation being 29.8%, including 18.6%) of acute lymphoblastic leukemia (ALL) and 41.4% of acute myeloeytic leukemia( AML). After a follow-up of 11 cases for 6 months, disappearance of N-ras gene mutation was found in 7 oases but continuous presence of N-ras gene mutation was found in 4 cases. However, no N-ras gene initiation was found in 50 normal healthy cases. Conclusion N-ras gene mutation is of im-portant significance in the prognosis of patients with acute leukemia.
中文关键词	N-ras基因 ; 急性白血 ; 病突变
英文关键词	N-ras gene acute leukemia mutation
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ONCOLOGY
WOS研究方向	Oncology
CSCD记录号	CSCD:1248713
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/203948
作者单位	1.第三军医大学附属新桥医院血液科, 重庆 400037, 中国; 2.第三军医大学基础医学部生物化学与分子生物学教研室, 重庆 400037, 中国
推荐引用方式 GB/T 7714	刘红,彭家和,钟小林,等. PCR-SSCP检测急性白血病患者N-ras基因突变及临床意义初探[J],2003,25(7):629-631.
APA	刘红,彭家和,钟小林,曹小林,张立,&何凤田.(2003).PCR-SSCP检测急性白血病患者N-ras基因突变及临床意义初探.第三军医大学学报,25(7),629-631.
MLA	刘红,et al."PCR-SSCP检测急性白血病患者N-ras基因突变及临床意义初探".第三军医大学学报 25.7(2003):629-631.