干旱区生态环境知识资源中心(Arid): 肝活检组织乙肝病毒核心抗原定量检测方法的建立

Arid

	肝活检组织乙肝病毒核心抗原定量检测方法的建立
其他题名	The Establishment of the Special-Qualitative-Quantitative-Analysis-Method of MBcAg for Liver Biopsy Tissue with CHB
	闫玉萍 1; 尹格平 2; 马继顺 3; 郭超英 1; 蒋雪梅 1; 朱彤宇 2
来源期刊	中华检验医学杂志
ISSN	1009-9158
出版年	2002
卷号	25 期号:1 页码:23-25
中文摘要	目的：探讨建立对乙肝活检组织乙肝病毒核心抗原（HBcAg)定性，定量检测的诊断方法。方法：将37例慢乙肝患者肝活检组织制备成单细胞悬液，用小鼠抗人HBcAg单克隆抗体标记后，应用流式细胞分析术进行了定性定量检测，并制定了诊断标准。结果：该方法对肝穿活检组织HBcAg定性检出率与临床常规乙型肝炎五项检验阳性检出的符合率为100％，病理学分期Ⅰ，Ⅱ期的慢乙肝肝活检组织HBcAg指数（HBcI)分别为：3．491．55，9．373．15，Ⅲ期的2例分别为：32．12和18．4，统计学证实肝穿活检组织HBcAg流式定量检测与慢乙肝肝活检组织病理分期显著相关（P＜0．05），且与治疗效果显著相关（P＜0．05，P＜0．01），能够作为一种检测疗效的方法。结论：该方法能够对HBcAg定性兼定量诊断，又能直接反映肝组织内乙肝病毒的存在，与目前较常规乙肝诊断方法相结合，对乙肝的诊治将有重要的意义。
英文摘要	Objective To investigate and establish the special qualitative and quantitative analysis method of HBcAg for liver biopsy tissue with CHB. Methods Thirty-seven patients with CHB were performed liver biopsy and the tissues were made cellular suspension, then immunologically marked by fluorescent monoantibody of HBcAb, at last qualitatively and quantitatively measured by Flow cytometry (FCM). Result This special qualitative analysis metliod of HBcAg for liver biopsy tissue with CHB was completely related to that of routine clinical test of HBcAg, the HBcAg I ndex (HBcI) by FCM of pathological I, II stages were respectively 3.49 1.55, 9.37 3.15, two oases of III stages were respectively 32.12 and 18.4, and the quantitative analysis was statistically correlated with the pathological staging and curative effect of CHB (P < 0.05). Conclusion This method could directly determinate the HBcAg in liver tissue. Combined with the routine clinical test of HBcAg, this method has important clinic significance.
中文关键词	乙型肝炎抗体 ; 流式细胞术 ; 针吸活组织检查
英文关键词	HBcAg CHB hepatitis B antibodies flow cytometry bipsy, neddle
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:1110349
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/203736
作者单位	1.济南市传染病医院肝炎肝, 250021; 2.济南军区总医院流式细胞研究室; 3.山东大学医学院传染病学教研室
推荐引用方式 GB/T 7714	闫玉萍,尹格平,马继顺,等. 肝活检组织乙肝病毒核心抗原定量检测方法的建立[J],2002,25(1):23-25.
APA	闫玉萍,尹格平,马继顺,郭超英,蒋雪梅,&朱彤宇.(2002).肝活检组织乙肝病毒核心抗原定量检测方法的建立.中华检验医学杂志,25(1),23-25.
MLA	闫玉萍,et al."肝活检组织乙肝病毒核心抗原定量检测方法的建立".中华检验医学杂志 25.1(2002):23-25.