干旱区生态环境知识资源中心(Arid): 杜氏利什曼原虫平原和荒漠疫区分离株LACK基因克隆及序列分析

Arid

	杜氏利什曼原虫平原和荒漠疫区分离株LACK基因克隆及序列分析
其他题名	Cloning and Sequence Analysis of LAK Gene of Leishmania Donovani Isolates from Plain and Desert Foci of China
	马莹; 胡孝素; 王雅静; 王子龙
来源期刊	中国寄生虫学与寄生虫病杂志
ISSN	1000-7423
出版年	2002
卷号	20 期号:4 页码:197-200
中文摘要	目的：测定我国平原疫区和荒漠疫区杜氏利什曼原虫分离株活性蛋白激酶C受体同源物（LACK）基因序列，并与山丘疫区分离株及国外利什曼原虫分离株进行比较。方法：应用RT－PCR扩增LACK基因，将其克隆入pUC18载体后用双脱氧链末端终止法测序，并与GenBank中相关数据进行比较。结果：用RT－PCR成功扩增出约950bp的LACK基因片段，测序结果表明其片段大小均为942bp，与GenBank中多种利什曼原虫LACK基因的核苷酸序列一致性达97％以上，我国山丘、平原和荒漠3个不同疫区杜氏利什曼原虫分离株的LACK基因序列的一致性达95％以上，结论：获得了我国平原和荒漠疫区杜氏利什曼原虫LACK基因序列，我国3个不同疫区杜氏利什曼原虫分离株的LACK基因具有高度同源性。
英文摘要	Objective To determine the nucleotide sequence of the LACK (Leishmania homologue of receptors for activated protein kinase C) gene of Leishmania donovani isolates from plain foci (L. d SD1) and desert foci (L. d XJ771) of China, and to find out the difference of the sequence of LACK gene with other Leishimania spp. and also the isolate from hill foci of China. Methods The LACK genes of L. d SD1 and L. d XJ771 were amplified by RT-PCR and cloned into pUC18 vector respectively, sequenced by the dideoxy chain termination method, then analyzed and compared with that of other isolates. Results The LACK genes of the two isolates were successfully cloned. Both of the 2 fragments were 942 bp in length. Comparison of the two nucleotide sequences with that of other Leishmania spp. in GenBank showed that the identities were more than 97%, and the identities of the nucleotide sequences of LACK genes of the three L. d isolates from plain, desert and hill foci of China were more than 95%. Conclusion High identities exist among the nucleotide sequences of LACK genes of the three L. d isolates from the three foci of China.
中文关键词	杜氏利什曼原虫 ; 荒漠疫区 ; 基因克隆 ; 序列分析 ; 平原疫区 ; 活性蛋白激酶C受体同源物 ; 对比分析
英文关键词	leishmania donovani LACK gene cloning gene sequencing
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:1088965
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/203621
作者单位	四川大学华西基础医学和法医学院寄生虫学教研室, 成都, 四川 610044, 中国
推荐引用方式 GB/T 7714	马莹,胡孝素,王雅静,等. 杜氏利什曼原虫平原和荒漠疫区分离株LACK基因克隆及序列分析[J],2002,20(4):197-200.
APA	马莹,胡孝素,王雅静,&王子龙.(2002).杜氏利什曼原虫平原和荒漠疫区分离株LACK基因克隆及序列分析.中国寄生虫学与寄生虫病杂志,20(4),197-200.
MLA	马莹,et al."杜氏利什曼原虫平原和荒漠疫区分离株LACK基因克隆及序列分析".中国寄生虫学与寄生虫病杂志 20.4(2002):197-200.