干旱区生态环境知识资源中心(Arid): 干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响

Arid

	干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响
其他题名	Effect of Drought Stress on Activities of Cell Defense Enzymes and Lipid Peroxidation in Glycyrrhiza uralensis Seedings
	李明; 王根轩
来源期刊	生态学报
ISSN	1000-0933
出版年	2002
卷号	22 期号:4 页码:503-507
中文摘要	甘草（Glycyrrhiza uralensis, fisch.）幼苗用PEG6000（-2Mpa,-1.5Mpa）模拟干旱处理，测定了4d中叶的MDA含量、膜相对透性及几种保护酶（SOD、POD、ASP、CAT）活性变化情况。结果表明：-2.5Mpa胁迫下，MDA含量总体呈上升趋势；-1.5Mpa胁迫下，前两天略有降低，至4天时，上升到与处理前基本持平的水平。细胞膜透性在处理前期下降，后期升高，低渗透胁迫较高渗透的胁迫变化平缓。几种保护酶活性在干旱处理期间都有变化：POD除第1天降低外，其余几天均呈上升趋势；在处理前期，SOD活性升高，CAT活性下降，而在后期其变化为SOD活性降低，CAT活性升高；ASP活性变化波动较大，-2.5Mpa胁迫下，第1、第3天有两次上升峰，第4天较处理前下降了104.3%。在-1.5Mpa胁迫下的几种保护酶活性变化的幅度较小。表明的高渗透胁迫能使膜脂过氧化而引起膜的损伤，低渗透胁迫程度对细胞膜脂过氧化及膜的透性影响较小，且可能对膜脂过氧化起到一定的防御作用。植物在干旱胁迫下保护酶系统的作用，可能是通过它们之间的相互协调且保持一个稳定的平衡态而进行的。
英文摘要	It is swell known that the drought stress may induce active oxygen species (AOS) generation and cause injuries to plants at various degrees. Defense enzymes such as SOD, POD and CAT etc, which clean AOS, exist in plant cells. AOS such as O~·_2、OH~.、H_2O_2 were cleansed effectively and the degrees of lipid peroxidation and cell membrane injure were decreased by the compatible functions of these enzymes. Liquorices (Glycyrrhiza uralensis) can survive in some extreme environmental conditions such as dehydration, high and low temperature, high sale or alkali concentrations in the arid or subarid areas. Liquorices are very important in hampering wind, fixing sand and improving soil construction, but it is known a little about the mechanism of its tolerance to drought stress. In this study, Liquorices seedlings were treated with the osmotic stress solution PEG 6000 (-2.5MPa, -1.5MPa). The content of MDA, cell membrane electrolyte leakage and several defense enzymes activities were measured at the different times after treatments. The content of MDA increased generally under -2.5MPa PEG stress, but decreased in the early stages of stress then increased to that of origirating state approximately under -1.5MPa PEG stress. The levels of the cell membrane electrolyte leakage were decreased in the early stages and increased in the later stages, the changed rate was slower under -1.5MPa PEG stress ,but decreased in the early stages of stress then increased to that of origrating state approximately under -1.5MPa PEG stress. The levels of the cell membrane electrolyte leakage were decreased in the early stages and increased in the later stages, the changed rate was slower under -1.5MPa stress than that under -2.5MPa stress. Several defense enzymes activities varied under -2.5MPa or -1.5MPa PEG osmotic stress; POD activities increased in all days except the first day; SOD activities raised and CAT activities declined, at 1 or 2d after treatment, but SOD activities decreased and CAT activities increased, ASP activities changed, which given a peak at the first and third day respectively under -2.5Mpa stress. All of the alterations were in less extent under -1.5Mpa stress than under -2.5Mpa correspondingly. This results suggested that the system of the defense enzymes might play a key role by balancing al of the responding enzymes under drought condition in plants.
中文关键词	甘草幼苗 ; 干旱胁迫 ; 保护酶 ; 丙二醛
英文关键词	drought stress liquorice seedlings cell defense enzyme MDA
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:1045161
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/203380
作者单位	兰州大学, 干旱农业国家重点实验室, 兰州, 甘肃 730000, 中国
推荐引用方式 GB/T 7714	李明,王根轩. 干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J]. 兰州大学,2002,22(4):503-507.
APA	李明,&王根轩.(2002).干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响.生态学报,22(4),503-507.
MLA	李明,et al."干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响".生态学报 22.4(2002):503-507.