干旱区生态环境知识资源中心(Arid): 塔拉组培技术研究

Arid

	塔拉组培技术研究
其他题名	A Study on the Tissue Culture of Tara (Caesalpinia spinosa Kuntze)
	李志国; 吴昊; 杨文云; 夏定久
来源期刊	林业科学研究
ISSN	1001-1498
出版年	2002
卷号	15 期号:4 页码:474-478
中文摘要	用塔拉嫩茎、茎尖、叶片、叶柄作外植体容易诱导形成愈伤组织，在MS + BA 1.5 mg·L~(-1) + 蔗糖30 g·L~(-1) + 琼脂5.7 g·L~(-1)培养基上芽苗分化率为50%。分别以1/2MS + BA 0.1 mg·L~(-1) + 蔗糖20 g·L~(-1) + 琼脂5.7 g·L~(-1)和MS + BA 1.5 mg·L~(-1) + IAA 0.2 mg·L~(-1) + 干酪素1.5 g·L~(-1) + 蔗糖30 g·L~(-1) + 琼脂5.7 g·L~(-1)作培养基，用胚轴诱导芽苗，愈伤组织块上的芽苗分化率79%。芽苗培育约20 d后，用1/2MS + NAA 3.0 mg·L~(-1) + IAA 1.0 mg·L~(-1) + KT 0.5 mg·L~(-1) + 蔗糖15 g·L~(-1) + 琼脂5.7 g·L~(-1)作生根培养基培养约15 d，生根率为76%。培养条件为：光照2000～2500 lx，温度25～29 ℃。
英文摘要	Tara, Caesalpina spinosa Kuntze, distributing naturally in South America, is mainly distributed in the valley area in the middle of the east Andes and in the seashore desert along the Pacific Ocean. The plant was successfully introduced to the xerothermic and semi-xerothermic valleys of Yunnan province, China in 1991. This paper deals with the technique of tissue culture of tara. There is rich tannin in tara explant because its tissue culture is very difficulty. Using tender stem, stem needle, leaf or leafstalk act as explant cultivate callus mass to cultivate callus mass and to use MS + BA 1.5 mg ·L~(-1) + cane sugar 30 g·L~(-1) + gagaragar 5.7 g·L~(-1) act as sprout polarization culture medium, the percentage of callus mass to sprout is only 50%. Using 1/2 MS + BA 0.1 mg·L~(-1) + cane sugar 20 g·L~(-1) + gagaragar 5.7 g·L~(-1) and MS + BA 1.5 mg·L~(-1) act as sprout polarization culture medium, the percentage of callus mass to sprout is only 50%. Using 1/2 MS + BA 0.1 mg·L~(-1） + cane sugar 30 g·L~(-1) + gagaragar 5.7 g·L~(-1) act as culture medium to culture asepsis young seedling 10 days and to culture callus mass about 20 days respectively, there are 79% callus mass that come from hypocotyl can be grows bud plantlets. To culture asepsis bud plantlet about 20 days, Using 1/2 MS + NAA 3.0 mg·L~(-1) + IAA 1.0 mg·L~(-1) + KT 0.5 mg·L~(-1) + cane sugar 15 g·L~(-1) + gagaragar 5.7 g·L~(-1) act as growing root culture medium, the cent of growing root bud plantlet is 76% after 15 days. The experiment condition is that illumination is 2000～2500 lx and the temperature is 25～29 ℃.
中文关键词	塔拉 ; 诱导生根
英文关键词	Tara (Caesalpinia spinosa Kuntze) tissue culture induced rooting
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FORESTRY
WOS研究方向	Forestry
CSCD记录号	CSCD:1036657
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/203307
作者单位	中国林业科学研究院资源昆虫研究所, 昆明, 云南 650216, 中国
推荐引用方式 GB/T 7714	李志国,吴昊,杨文云,等. 塔拉组培技术研究[J],2002,15(4):474-478.
APA	李志国,吴昊,杨文云,&夏定久.(2002).塔拉组培技术研究.林业科学研究,15(4),474-478.
MLA	李志国,et al."塔拉组培技术研究".林业科学研究 15.4(2002):474-478.