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Salix psammophila can grow rapidly in desert and grassland areas. As an abundant bioresource, it is useful to understand its cell morphology, chemical compositions and mechanical properties. In this study, Anatomical properties of Salix psammophila in different annual rings were measured and compared. Fourier transform infrared spectroscopy and chemical titration were used to estimate cell wall chemical compositions. Moreover, mechanical properties in different annual rings were measured through nanoindentation. Fiber cell lumen diameter, fiber cell wall thickness, vessel cell lumen diameter and vessel cell wall thickness of Salix psammophila were measured to be 7.371, 2.285, 32.541 and 1.926 mu m, respectively. Fiber cell lumen diameter, fiber cell wall thickness, vessel cell lumen diameter differs among the annual rings. The cellulose, hemicellulose and lignin contents of Salix psammophila were 44.43, 34.99 and 17.93 %, respectively. Both hemicellulose and lignin contents varied among the annual rings with more hemicellulose but less lignin at the annual ring closer to the pith. The modulus of elasticity (MOE) of fiber cell wall of Salix psammophila decreases from pith to bark.