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In vitro culture and rooting of stem cuttings can be used for vegetative propagation of plant material. Argan trees (Argania spinosa (L.) Skeels) are endemic to semi-desert areas of South-western Morocco, where they cover more than 8000 km(2) and have important socio-economic and environmental impacts. Seed germination was improved by culturing in vitro isolated kernels on Murashige and Skoog (MS) axenic medium with 1.0 mg/L gibberellic acid (GA3). Newly in vitro-produced seedlings were then transferred to MS medium containing 0.5 mg/L thidiazuron (TDZ) and 1.0 mg/L GA3 to promote shoot development. The microcuttings produced were rooted using MS medium supplemented with two synthetic auxins, 1-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA), at 1.0 mg/L each. The rooted microcuttings were then transplanted to soil and acclimated in a greenhouse environment. In addition, hardwood stem cuttings collected from adult argan trees were rooted by using a combination of IBA and NAA.