Arid
DOI10.1590/18069657rbcs20160302
Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil
Souza Menezes, Kelly Alexsandra1; Costa Escobar, Indra Elena2; Resende Fraiz, Ana Carla2; Vieira Martins, Lindete Miria3; Fernandes-Junior, Paulo Ivan4
通讯作者Fernandes-Junior, Paulo Ivan
来源期刊REVISTA BRASILEIRA DE CIENCIA DO SOLO
ISSN0100-0683
出版年2017
卷号41
英文摘要

Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from "mulungu" (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments. The first assay was carried out under gnotobiotic conditions, with sterile sand as a substrate; the second experiment was conducted in a non-sterile soil. For both experiments, the inoculation treatments consisted of a single inoculation of each isolate, in addition to a treatment with Bradyrhizobium elkanii BR 5609 as a reference strain. Furthermore, two non-inoculated control treatments, supplied and not supplied with mineral N, were also evaluated. Bacterial identification indicated that both alpha and beta-rhizobia could be found in "mulungu" root nodules. Three isolates where classified within the Rhizobium genus, four bacteria belonged to Bradyrhizobium and one isolate clustered with Burkholderia. Positive amplification of an intragenic fragment of the nodA gene using a primer set to beta-rhizobia could be found for ESA 71 (Burkholderia). All bacterial isolates were effective in colonizing "mulungu" roots. In the first experiment, all inoculated treatments and N fertilization increased the N concentration in "mulungu" shoot tissues. For total N in the shoots, the isolates ESA 70, ESA 72, and ESA 75 stood out. In the non-sterile substrate experiment, the isolates ESA 70, ESA 71, ESA 72, and ESA 75, together with the reference strains, induced increases in the shoot N concentration and total accumulation compared to the absolute control. The results indicate that "mulungu" is able to establish associations with efficient alpha and beta-rhizobia in Brazilian semi-arid soils.


英文关键词biological nitrogen fixation "mulungu" tree legumes "Caatinga" rhizobia
类型Article
语种英语
国家Brazil
收录类别SCI-E
WOS记录号WOS:000394157700006
WOS关键词RHIZOBIA ; LEGUMES ; DIVERSITY ; CAATINGA ; STRAINS ; SPP.
WOS类目Soil Science
WOS研究方向Agriculture
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/202045
作者单位1.Univ Estado Bahia, Dept Tecnol & Ciencias Sociais, Programa Posgrad Horti Irrigada, Juazeiro, BA, Brazil;
2.Univ Fed Vale Sao Francisco, Colegiado Farmac, Programa Posgad Recursos Nat Semiarido, Petrolina, PE, Brazil;
3.Univ Estado Bahia, Dept Tecnol & Ciencias Sociais, Juazeiro, BA, Brazil;
4.Empresa Brasileira Pesquisa Agr, Embrapa Semiarido, Petroiina, PE, Brazil
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Souza Menezes, Kelly Alexsandra,Costa Escobar, Indra Elena,Resende Fraiz, Ana Carla,et al. Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil[J],2017,41.
APA Souza Menezes, Kelly Alexsandra,Costa Escobar, Indra Elena,Resende Fraiz, Ana Carla,Vieira Martins, Lindete Miria,&Fernandes-Junior, Paulo Ivan.(2017).Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil.REVISTA BRASILEIRA DE CIENCIA DO SOLO,41.
MLA Souza Menezes, Kelly Alexsandra,et al."Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil".REVISTA BRASILEIRA DE CIENCIA DO SOLO 41(2017).
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