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Camels from Cholistan desert of Pakistan were studied for Staphylococcus aureus mastitis infection, associated risk factors and subsequently their antimicrobial susceptibility. The milk samples were screened with surf field mastitis test and furthered to biochemical analysis. Pearson’ Chi-square test at nighty five percent confidence interval was used to analyze the collected data. Antibiotic sensitivity was checked with twenty four antibiotics by disc diffusion method. Over all mastitis was found 52.5% (63/120) with leading subclinical (41.67%, 50/120) form of mastitis on overall milk collected data. Pathogen type was crowned by Staph. aureus with 74.5% (47/63) prevalence, following which were Streptococcus species (17.45%, 11/63), E. colt (3.17%, 2/63), and Bacillus cereus (4.76%, 3/63). Coagulase positive Staph. aureus (41 out of 47), and hemolysin producing Staph. aureus (39 out of 47) primed among Staph. aureus isolates indicating very pathogenic nature of infection in the area. Risk factors determinants were found significantly (P < 0.05) associated with mastitis occurrence except frequency of milking per day. Antibiogram of Staph. aureus indicated very strong resistance to oxacillin, ticarcillin, ampicilline, amoxicillin, azlocine, chloramphenicol, mupirocin, vancomycin, cefixinme, cefuroxime, and cefotaxime. In contrast to this sulphaphenazole, gentamicin, amikacin, and ciprofloxacin were highly sensitive. Piperacillin, Tazabactam and cinxacin were moderately effective against Staph. aureus. The concluded remarks of research staged Staph. aureus to the most obvious pathogen and widely resistant to antimicrobials camel mastitogen. The risk factors were found soul determinants of pathogen spread among mammary glands of camels.