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Roots of Rhodiola imbricata Edgew from Indian trans-Himalayan cold desert known for their nutritional and medicinal attributes were evaluated for the dietary amino acids, fatty acids and mineral composition. Nine essential and twelve non-essential amino acids were quantified. The contents ranged between 91.33 and 1640.67 A mu g/g. Histidine (1434.33 A mu g/g), lysine (1329.33 A mu g/g) and threonine (1015.67 mu g/g) were dominant essential amino acids, while glycine (1640.67 A mu g/g), proline (1263.67 A mu g/g), alanine (1142.33 A mu g/g), cystine HCL (1136.33 mu g/g) and nor leucine (1038.67 mu g/g) were major non essential amino acids. The total lipid was found to be rich source of saturated fatty acids such as capric acid (19.91%), caproic acid (10.87%), palmitic acid (9.42%), lignoceric acid (6.16%) and behenic acid (5.71%), which together constituted 52% of the lipid content. Linoleic acid (15.06%), oleic acid (12.38%), arachidonic acid (8.38%), linolelaidic acid (6.11%) and docosadienoic acid (5.99%) were prominent unsaturated fatty acids (UFAs). Mono unsaturated fatty acids (MUFAs) and poly unsaturated fatty acids (PUFAs) were 35.64% and 12.33% of the lipid content respectively. Calcium (11034.17 mg/kg), potassium (2143.25 mg/kg), iron (1441.17 mg/kg), magnesium (581.99 mg/kg), phosphorous (376.72 mg/kg) and sodium (109.75 mg/kg) were detected as the major dietary minerals.