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A Gram-stain-negative, rod-shaped, non-motile bacterium, designated D4N98(T), was isolated from a desert soil near Glendambo, Australia. The taxonomic position of strain D4N98(T) was investigated using a polyphasic approach. Strain D4N98(T) shared 97% 16S rRNA gene sequence similarity with the only reference strain of the genus Izhakiella (Izhakiella capsodis N6PO6(T)), and less than 96% similarity with other species of genera in the family Enterobacteriaceae with validly published names. Phylogenetic analysis, based on 16S rRNA, rpoB, atpD, gyrB genes and a concatenated sequence comprising 37 single copy marker genes, as well as chemotaxonomic data (major polar lipids: phosphatidyl ethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a neutral lipid, and a glycolipid. Major fatty acids (> 10 %): C-16 : 0, C-17 : 0 cyclo, summed feature 2 (C-14 : 0 3-OH and/or iso-C-16 : 1 I), summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c), summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c) support the affiliation of this strain to the genus Izhakiella. The results of in silico DNA-DNA hybridisation plus physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain D4N98(T) from the other species of the genus Izhakiella with validly published names. Therefore, strain D4N98(T) represents a novel species, for which the name Izhakiella australiensis sp. nov. is proposed. The type strain is D4N98(T) (LMG 30066(T) = DSM 105030(T)).