干旱区生态环境知识资源中心

A Gram-stain-positive actinobacterium, designated as strain 2-36(T), was isolated from sludge of a sewage outlet in a manganese mine. Phylogenetic analysis of 16S rRNA genes assigned strain 2-36(T) in a single lineage in the family Geodermatophilaceae and closely related to Modestobacter roseus KLBMP 1279(T) (93.8% similarity), Blastococcus saxobsidens BC444(T) (93.1 %) and Geodermatophilus aquaeductus BMG801(T) (92.8 %). It contained iso-C-16 : 0, iso-C-15 : 0, C-17 : 1 omega 6c, iso-C-14 : 0 and summed feature 3 (C-16 : 1 omega 6c and/or C-16 : 1 omega 7c) as the major fatty acids (> 5 %), with MK-9(H-4), MK-8(H-4) and MK-9(H-6) as the quinones. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid and the DNA G+C content was 70.1 mol%. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unknown glycolipid, an unknown aminophospholipid and two unknown phospholipids. Compared to closely related strains, strain 2-36(T) showed distinguishing characteristics, such as the distinct phylogenetic lineage, positive result for phenylacetic acid assimilation and negative result for alpha-glucosidase and having C-17 : 1 omega 6c and iso-C-14 : 0 as the major fatty acids. On the basis of the polyphasic analyses, strain 2-36(T) represents a novel species of a new genus within the family Geodermatophilaceae, for which the name Cumulibacter manganitolerans gen. nov., sp. nov. is proposed. The type strain of ’Cumulibacter manganitolerans’ is 2-36(T) (=CCTCC AA 2016026(T) =DSM 103787(T)).