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DOI | 10.7150/ijbs.21976 |
The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair | |
Wang, Yang1; Li, Dong2; Wang, Gangyang1; Chen, Lulu3,4; Chen, Jun1; Liu, Zhangyin5; Zhang, Zhaofeng1; Shen, Hua1; Jin, Yuqing1; Shen, Zunli1 | |
通讯作者 | Shen, Hua ; Jin, Yuqing ; Shen, Zunli |
来源期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
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ISSN | 1449-2288 |
出版年 | 2017 |
卷号 | 13期号:12页码:1507-1519 |
英文摘要 | Combinations of fibroblasts (Fbs) and corresponding epithelial cells have been widely used in many tissues, such as the skin and breast tissues, to augment tissue repair and remodeling. Recently, a large amount of new data has indicated that nerve Fbs play critical roles in Schwann cells (SCs) and axons in vitro. However, little is known regarding the effects of co-transplanting nerve Fbs and SCs on peripheral nerve repair in vivo. The aim of this study was to investigate the effect of co-transplanting sciatic nerve Fbs (SN-Fbs) and sciatic nerve SCs (SN-SCs) on nerve regeneration. We developed a 5 mm nerve-defect model in mice using a polyurethane (PUR) catheter and then injected one of four different mixtures of cells into the catheters to form the following four groups: pure Matrigel (Control group), SN-Fbs alone (SN-Fb group), SN-Fbs combined with SN-SCs at a ratio of 1:2 (Fb&SC group) and SN-SCs alone (SN-SC group). Histological and functional analyses were performed 3 months later. The results indicated that in vitro, the expression levels of NGF, BDNF and GDNF were significantly higher, and in vivo, a more moderate amount of extracellular matrix was produced in the Fb&SC group than in the SN-SC group. Compared to the other groups, co-transplanting SN-Fbs with SCs at a 1:2 ratio had significantly positive effects on nerve regeneration and functional recovery. |
英文关键词 | fibroblasts Schwann cells |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000418365000005 |
WOS关键词 | BASAL LAMINA DEPOSITION ; STEM-CELLS ; ENDONEURIAL FIBROBLASTS ; EPIDERMAL REGENERATION ; DESERT HEDGEHOG ; CONDUIT ; DIFFERENTIATION ; MYELINATION ; MIGRATION ; PROMOTES |
WOS类目 | Biochemistry & Molecular Biology ; Biology |
WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/199708 |
作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Plast & Reconstruct Surg, Sch Med, Shanghai, Peoples R China; 2.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Sch Med, Shanghai, Peoples R China; 3.Chinese Acad Med Sci, Plast Surg Hosp, Beijing, Peoples R China; 4.Peking Union Med Coll, Beijing, Peoples R China; 5.Jiangpu Primary Hlth Serv Ctr, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Yang,Li, Dong,Wang, Gangyang,et al. The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair[J],2017,13(12):1507-1519. |
APA | Wang, Yang.,Li, Dong.,Wang, Gangyang.,Chen, Lulu.,Chen, Jun.,...&Shen, Zunli.(2017).The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,13(12),1507-1519. |
MLA | Wang, Yang,et al."The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 13.12(2017):1507-1519. |
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