Arid
DOI10.1016/j.ibmb.2017.09.011
Accumulation of dsRNA in endosomes contributes to inefficient RNA interference in the fall armyworm, Spodoptera frugiperda
Yoon, June-Sun; Gurusamy, Dhandapani; Palli, Subba Reddy
通讯作者Palli, Subba Reddy
来源期刊INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN0965-1748
EISSN1879-0240
出版年2017
卷号90页码:53-60
英文摘要

RNA interference (RNAi) efficiency varies among insects studied. The barriers for successful RNAi include the presence of double-stranded ribonucleases (dsRNase) in the lumen and hemolymph that could potentially digest double-stranded RNA (dsRNA) and the variability in the transport of dsRNA into and within the cells. We recently showed that the dsRNAs are transported into lepidopteran cells, but they are not processed into small interference RNAs (siRNAs) because they are trapped in acidic bodies. In the current study, we focused on the identification of acidic bodies in which dsRNAs accumulate in Sf9 cells. Time-lapse imaging studies showed that dsRNAs enter Sf9 cells and accumulate in acidic bodies within 20 min after their addition to the medium. CypHer-5E-labeled dsRNA also accumulated in the midgut and fat body dissected from Spodoptera frugiperda larvae with similar patterns observed in Sf9 cells. Pharmacological inhibitor assays showed that the dsRNAs use clathrin mediated endocytosis pathway for transport into the cells. We investigated the potential dsRNA accumulation sites employing LysoTracker and double labeling experiments using the constructs to express a fusion of green fluorescence protein with early or late endosomal marker proteins and CypHer-5E-labeled dsRNA. Interestingly, CypHer-5E-labeled dsRNA accumulated predominantly in early and late endosomes. These data suggest that entrapment of internalized dsRNA in endosomes is one of the major factors contributing to inefficient RNAi response in lepidopteran insects. (C) 2017 Elsevier Ltd. All rights reserved.


英文关键词RNAi Endosomes Double-stranded RNA Lepidoptera
类型Article
语种英语
国家USA
收录类别SCI-E
WOS记录号WOS:000415908900006
WOS关键词DOUBLE-STRANDED-RNA ; COLORADO POTATO BEETLE ; CAENORHABDITIS-ELEGANS ; LEPTINOTARSA-DECEMLINEATA ; SCHISTOCERCA-GREGARIA ; DESERT LOCUST ; INSECT PESTS ; IDENTIFICATION ; DEGRADATION ; EXPRESSION
WOS类目Biochemistry & Molecular Biology ; Entomology
WOS研究方向Biochemistry & Molecular Biology ; Entomology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/199653
作者单位Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA
推荐引用方式
GB/T 7714
Yoon, June-Sun,Gurusamy, Dhandapani,Palli, Subba Reddy. Accumulation of dsRNA in endosomes contributes to inefficient RNA interference in the fall armyworm, Spodoptera frugiperda[J],2017,90:53-60.
APA Yoon, June-Sun,Gurusamy, Dhandapani,&Palli, Subba Reddy.(2017).Accumulation of dsRNA in endosomes contributes to inefficient RNA interference in the fall armyworm, Spodoptera frugiperda.INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,90,53-60.
MLA Yoon, June-Sun,et al."Accumulation of dsRNA in endosomes contributes to inefficient RNA interference in the fall armyworm, Spodoptera frugiperda".INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 90(2017):53-60.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yoon, June-Sun]的文章
[Gurusamy, Dhandapani]的文章
[Palli, Subba Reddy]的文章
百度学术
百度学术中相似的文章
[Yoon, June-Sun]的文章
[Gurusamy, Dhandapani]的文章
[Palli, Subba Reddy]的文章
必应学术
必应学术中相似的文章
[Yoon, June-Sun]的文章
[Gurusamy, Dhandapani]的文章
[Palli, Subba Reddy]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。