Arid
DOI10.1080/01490451.2016.1238980
Comparative Analysis of 16S rRNA Gene Illumina Sequence for Microbial Community Structure in Diverse Unexplored Hot Springs of Odisha, India
Sahoo, Rajesh Kumar1; Gaur, Mahendra1; Das, Aradhana1; Singh, Archana1; Kumar, Mohit2; Subudhi, Enketeswara1
通讯作者Kumar, Mohit ; Subudhi, Enketeswara
来源期刊GEOMICROBIOLOGY JOURNAL
ISSN0149-0451
EISSN1521-0529
出版年2017
卷号34期号:6页码:567-576
英文摘要

Odisha (East India) is home to several hot springs, of which Atri and Taptapani are the two with variation in temperature and located in the Mahanadi geothermal province having altitude 120 and 1800ft., respectively, above sea level. Average temperature of Atri hot spring is as higher as 58 +/- 5 degrees C as compared to 48 +/- 5 degrees C of Taptapani. In-depth analysis of the microbial diversity of these hot springs through 16S rRNA deep sequencing analysis, targeting V3 region was performed using Illumina bar-coded sequencing platform. Existence of higher microbial diversity in Atri hot spring (1662 OTUs; 2708 species) as compared to Taptapani (1561 Operational Taxonomic Units [OTUs]; 2045 species) is supported by higher value of diversity indices for Atri (6.24, Shannon; 0.95, Simpson) than Taptapani (4.03, Shannon; 0.79, Simpson), probably due to favorable influence of environmental parameters around it. Irrespective of the four databases (GREENGENE, M5RNA, Ribosomal Database Project [RDP], and Small Subunit [SSU] databases) used for understanding community structure, the dominant phyla in the Atri hot spring were different from the predominant populations in the Taptapani in terms of percentage representation in different databases. From Principal Coordinates Analysis [PCoA] analysis, Atri and Taptapani metagenome, on comparison with other three metagenomes, were found to be matching with the community structure of hot springs of Gujarat, India, but differed from that of saline desert. Furthermore, predicted functional analysis in both the hot springs were found to be affiliated with carbohydrate, amino acids, energy, vitamins and cofactor, nucleotide, membrane transport metabolic pathways, and the genes involved in them, although their intensity of occurrence was varying as analyzed through PICRUSt and Tax4Fun probably due to physicochemical parameters prevailing around each hot spring. The present study for the first time has revealed the differential microbial community structure and predicted functional diversity of Atri and Taptapani hot springs of Odisha in such a great detail.


英文关键词Bacterial community functional analysis hot springs metagenomes
类型Article
语种英语
国家India
收录类别SCI-E
WOS记录号WOS:000401743100009
WOS关键词BACTERIAL DIVERSITY ; TEMPERATURE ; BIODIVERSITY ; SEDIMENTS ; MATS ; PH
WOS类目Environmental Sciences ; Geosciences, Multidisciplinary
WOS研究方向Environmental Sciences & Ecology ; Geology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/199254
作者单位1.Siksha O Anusandhan Univ, Ctr Biotechnol, Bhubaneswar 751003, Odisha, India;
2.Mahatma Jyoti Rao Phoole Univ, Dept Biotechnol, Jaipur, Rajasthan, India
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Sahoo, Rajesh Kumar,Gaur, Mahendra,Das, Aradhana,et al. Comparative Analysis of 16S rRNA Gene Illumina Sequence for Microbial Community Structure in Diverse Unexplored Hot Springs of Odisha, India[J],2017,34(6):567-576.
APA Sahoo, Rajesh Kumar,Gaur, Mahendra,Das, Aradhana,Singh, Archana,Kumar, Mohit,&Subudhi, Enketeswara.(2017).Comparative Analysis of 16S rRNA Gene Illumina Sequence for Microbial Community Structure in Diverse Unexplored Hot Springs of Odisha, India.GEOMICROBIOLOGY JOURNAL,34(6),567-576.
MLA Sahoo, Rajesh Kumar,et al."Comparative Analysis of 16S rRNA Gene Illumina Sequence for Microbial Community Structure in Diverse Unexplored Hot Springs of Odisha, India".GEOMICROBIOLOGY JOURNAL 34.6(2017):567-576.
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