Arid
DOI10.3389/fpls.2017.01469
CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing
Sattar, Muhammad N.1; Iqbal, Zafar2; Tahir, Muhammad N.3; Shahid, Muhammad S.4; Khurshid, Muhammad5; Al-Khateeb, Abdullatif A.6; Al-Khateeb, Suliman A.1,7
通讯作者Sattar, Muhammad N.
来源期刊FRONTIERS IN PLANT SCIENCE
ISSN1664-462X
出版年2017
卷号8
英文摘要

The genetic modifications through breeding of crop plants have long been used to improve the yield and quality. However, precise genome editing (GE) could be a very useful supplementary tool for improvement of crop plants by targeted genome modifications. Various GE techniques including ZFNs (zinc finger nucleases), TALENs (transcription activator-like effector nucleases), and most recently clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 (CRISPR-associated protein 9)based approaches have been successfully employed for various crop plants including fruit trees. CRISPR/Cas9-based approaches hold great potential in GE due to their simplicity, competency, and versatility over other GE techniques. However, to the best of our knowledge no such genetic improvement has ever been developed in date palm-an important fruit crop in Oasis agriculture. The applications of CRISPR/Cas9 can be a challenging task in date palm GE due to its large and complex genome, high rate of heterozygosity and outcrossing, in vitro regeneration and screening of mutants, high frequency of single-nucleotide polymorphism in the genome and ultimately genetic instability. In this review, we addressed the potential application of CRISPR/Cas9-based approaches in date palm GE to improve the sustainable date palm production. The availability of the date palm whole genome sequence has made it feasible to use CRISPR/Cas9 GE approach for genetic improvement in this species. Moreover, the future prospects of GE application in date palm are also addressed in this review.


英文关键词CRISPR/Cas9 date palm genome editing multiplexing loss of and gain-of-functions
类型Review
语种英语
国家Saudi Arabia ; Pakistan ; Oman
收录类别SCI-E
WOS记录号WOS:000408212800004
WOS关键词TARGETED MUTAGENESIS ; PAIRED NICKASES ; HOMOLOGOUS RECOMBINATION ; CRISPR-CAS9 NUCLEASES ; RAPID GENERATION ; TRANSFER-RNA ; GUIDE-RNA ; GENE ; ARABIDOPSIS ; CAS9
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/199102
作者单位1.King Faisal Univ, Fac Agr & Food Sci, Dept Environm & Nat Resources, Al Hasa, Saudi Arabia;
2.Akhuwat Faisalabad Inst Res Sci & Technol, Faisalabad, Pakistan;
3.Natl Inst Biotechnol & Genet Engn, Faisalabad, Pakistan;
4.Sultan Qaboos Univ, Coll Agr & Marine Sci, Dept Crop Sci, Al Khoud, Oman;
5.Univ Punjab, Inst Biochem & Biotechnol, Lahore, Pakistan;
6.King Faisal Univ, Fac Agr & Food Sci, Plant Biotechnol Dept, Al Hasa, Saudi Arabia;
7.Minist Environm Water & Agr, Riyadh, Saudi Arabia
推荐引用方式
GB/T 7714
Sattar, Muhammad N.,Iqbal, Zafar,Tahir, Muhammad N.,et al. CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing[J],2017,8.
APA Sattar, Muhammad N..,Iqbal, Zafar.,Tahir, Muhammad N..,Shahid, Muhammad S..,Khurshid, Muhammad.,...&Al-Khateeb, Suliman A..(2017).CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing.FRONTIERS IN PLANT SCIENCE,8.
MLA Sattar, Muhammad N.,et al."CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing".FRONTIERS IN PLANT SCIENCE 8(2017).
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