Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.3389/fpls.2017.00216 |
Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns | |
Morales, Andrea1,4; Zurita-Silva, Andres2; Herman, Jonathan Maldonado3; Silva, Herman3 | |
通讯作者 | Zurita-Silva, Andres ; Silva, Herman |
来源期刊 | FRONTIERS IN PLANT SCIENCE
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ISSN | 1664-462X |
出版年 | 2017 |
卷号 | 8 |
英文摘要 | Global freshwater shortage is one of the biggest challenges of our time, often associated to misuse, increased consumption demands and the effects of climate change, paralleled with the desertification of vast areas. Chenopodium quinoa (Willd.) represents a very promising species, due to both nutritional content and cultivation under water constraint. We characterized drought tolerance of three Chilean genotypes and selected Genotype R49 (Salares ecotype) based upon Relative Water Content (RWC), Electrolyte Leakage (EL) and maximum efficiency of photosystem II (F-v/F-m) after drought treatment, when compared to another two genotypes. Exploratory RNA-Seq of R49 was generated by Illumina paired-ends method comparing drought and control irrigation conditions. We obtained 104.8 million reads, with 54 million reads for control condition and 51 million reads for drought condition. Reads were assembled in 150,952 contigs, were 31,523 contigs have a reading frame of at least 300 nucleotides (100 aminoacids). BLAST2GO annotation showed a 15% of genes without homology to NCBI proteins, but increased to 19% (306 contigs) when focused into drought-induced genes. Expression pattern for canonical drought responses such as ABA biosynthesis and other genes induced were assessed by qPCR, suggesting novelty of R49 drought responses. |
英文关键词 | drought tolerance Andean grain Salares and coastal/lowlands genotypes RNA-Seq qPCR |
类型 | Article |
语种 | 英语 |
国家 | Chile |
收录类别 | SCI-E |
WOS记录号 | WOS:000395519200001 |
WOS关键词 | HEAT-SHOCK PROTEINS ; GENE-EXPRESSION ; STRESS-RESPONSE ; DROUGHT TOLERANCE ; SEQUENCE TAGS ; SALINITY ; ARABIDOPSIS ; COLD ; GENOME ; RNA |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/199084 |
作者单位 | 1.Univ La Serena, Ctr Estudios Avanzados Zonas Aridas, La Serena, Chile; 2.Inst Invest Agr, Ctr Invest Intihuasi, La Serena, Chile; 3.Univ Chile, Fac Ciencias Agron, Dept Prod Agr, Lab Genom Func & Bioinformat, Santiago, Chile; 4.Univ Santo Tomas, Fac Salud Sede La Serena, Escuela Tecnol Med, Santiago, Chile |
推荐引用方式 GB/T 7714 | Morales, Andrea,Zurita-Silva, Andres,Herman, Jonathan Maldonado,et al. Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns[J],2017,8. |
APA | Morales, Andrea,Zurita-Silva, Andres,Herman, Jonathan Maldonado,&Silva, Herman.(2017).Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns.FRONTIERS IN PLANT SCIENCE,8. |
MLA | Morales, Andrea,et al."Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns".FRONTIERS IN PLANT SCIENCE 8(2017). |
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