Arid
DOI10.3389/fpls.2017.00216
Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns
Morales, Andrea1,4; Zurita-Silva, Andres2; Herman, Jonathan Maldonado3; Silva, Herman3
通讯作者Zurita-Silva, Andres ; Silva, Herman
来源期刊FRONTIERS IN PLANT SCIENCE
ISSN1664-462X
出版年2017
卷号8
英文摘要

Global freshwater shortage is one of the biggest challenges of our time, often associated to misuse, increased consumption demands and the effects of climate change, paralleled with the desertification of vast areas. Chenopodium quinoa (Willd.) represents a very promising species, due to both nutritional content and cultivation under water constraint. We characterized drought tolerance of three Chilean genotypes and selected Genotype R49 (Salares ecotype) based upon Relative Water Content (RWC), Electrolyte Leakage (EL) and maximum efficiency of photosystem II (F-v/F-m) after drought treatment, when compared to another two genotypes. Exploratory RNA-Seq of R49 was generated by Illumina paired-ends method comparing drought and control irrigation conditions. We obtained 104.8 million reads, with 54 million reads for control condition and 51 million reads for drought condition. Reads were assembled in 150,952 contigs, were 31,523 contigs have a reading frame of at least 300 nucleotides (100 aminoacids). BLAST2GO annotation showed a 15% of genes without homology to NCBI proteins, but increased to 19% (306 contigs) when focused into drought-induced genes. Expression pattern for canonical drought responses such as ABA biosynthesis and other genes induced were assessed by qPCR, suggesting novelty of R49 drought responses.


英文关键词drought tolerance Andean grain Salares and coastal/lowlands genotypes RNA-Seq qPCR
类型Article
语种英语
国家Chile
收录类别SCI-E
WOS记录号WOS:000395519200001
WOS关键词HEAT-SHOCK PROTEINS ; GENE-EXPRESSION ; STRESS-RESPONSE ; DROUGHT TOLERANCE ; SEQUENCE TAGS ; SALINITY ; ARABIDOPSIS ; COLD ; GENOME ; RNA
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/199084
作者单位1.Univ La Serena, Ctr Estudios Avanzados Zonas Aridas, La Serena, Chile;
2.Inst Invest Agr, Ctr Invest Intihuasi, La Serena, Chile;
3.Univ Chile, Fac Ciencias Agron, Dept Prod Agr, Lab Genom Func & Bioinformat, Santiago, Chile;
4.Univ Santo Tomas, Fac Salud Sede La Serena, Escuela Tecnol Med, Santiago, Chile
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GB/T 7714
Morales, Andrea,Zurita-Silva, Andres,Herman, Jonathan Maldonado,et al. Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns[J],2017,8.
APA Morales, Andrea,Zurita-Silva, Andres,Herman, Jonathan Maldonado,&Silva, Herman.(2017).Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns.FRONTIERS IN PLANT SCIENCE,8.
MLA Morales, Andrea,et al."Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns".FRONTIERS IN PLANT SCIENCE 8(2017).
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