Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.3389/fphar.2017.00232 |
In Vitro and In Silico Antidiabetic and Antimicrobial Evaluation of Constituents from Kickxia ramosissima (Nanorrhinum ramosissimum) | |
Amin, Adnan1; Tuenter, Emmy1; Foubert, Kenn1; Iqbal, Jamhsed2; Cos, Paul3; Maes, Louis3; Exarchou, Vassiliki1; Apers, Sandra1; Pieters, Luc1 | |
通讯作者 | Pieters, Luc |
来源期刊 | FRONTIERS IN PHARMACOLOGY |
ISSN | 1663-9812 |
出版年 | 2017 |
卷号 | 8 |
英文摘要 | Background and Aims: Kickxia ramosissima (Wall.) Janch (or Nanorrhinum ramosissimum (Wall.) Betsche is a well-known medicinal plant in Pakistan that is traditionally used in diabetic and inflammatory conditions. Because little information is available on its phytochemical composition, a range of constituents were isolated and evaluated in vitro in assays related to the traditional use. Methods: Dried whole plant material was extracted and chromatographically fractionated. Isolated constituents were evaluated in silico and in vitro in assays related to the traditional use against diabetes (inhibition of alpha-glucosidase activity; inhibition of advanced glycation endproducts) and in inflammatory conditions (inhibition of AAPH induced linoleic acid peroxidation, inhibition of 15-LOX, antimicrobial activity). Results: Phytochemical analysis of the extracts and fractions led to isolation of 7 compounds, including the iridoids kickxiasine (being a new compound), mussaenosidic acid, mussaenoside and linarioside; the flavonoids pectolinarigenin and pectolinarin; and 4-hydroxy-benzoic acid methyl ester. The iridoids showed weak antiglycation activity. The flavonoids, however, showed interesting results as pectolinarigenin was highly active compared to pectolinarin. In the alpha-glucosidase inhibition assay, only weak activity was observed for the iridoids. However, the flavonoid pectolinarigenin showed good activity, followed by pectolinarin. In the 15-LOX experiment, moderate inhibition was recorded for most compounds, the iridoids mussaenosidic acid and mussaenoside being the most active. In the AAPH assay, weak or no inhibition was recorded for all compounds. The in silico assays for the alpha-glucosidase and 15-LOX assays confirmed the results of respective in vitro assays. Pectolinarigenin showed moderate antimicrobial activity against Staphylococcus aureus, Plasmodium falciparum K1, and Trypanosoma cruzi, but it was not cytotoxic on a human MRC-5 cell line. Conclusion: Our findings may in part contribute to explain the traditional use of K. ramosissima. |
英文关键词 | Kickxia ramosissima Nanorrhinum ramosissimum Plantaginaceae iridoids antimicrobial activity antiglycation activity |
类型 | Article |
语种 | 英语 |
国家 | Belgium ; Pakistan |
收录类别 | SCI-E |
WOS记录号 | WOS:000400635000001 |
WOS关键词 | GLUCOSIDASE INHIBITORY-ACTIVITIES ; IRIDOID GLUCOSIDES ; ALPHA-GLUCOSIDASE ; CHEMICAL-CONSTITUENTS ; QUANTITATIVE-ANALYSIS ; REHMANNIA-GLUTINOSA ; LINARIA-JAPONICA ; LEAF EXTRACTS ; NARA DESERT ; FLAVONOIDS |
WOS类目 | Pharmacology & Pharmacy |
WOS研究方向 | Pharmacology & Pharmacy |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/199068 |
作者单位 | 1.Univ Antwerp, Lab Nat Prod & Food Res & Anal, Dept Pharmaceut Sci, Antwerp, Belgium; 2.COMSATS Inst Informat Technol, Ctr Adv Drug Res, Abbottabad, Pakistan; 3.Univ Antwerp, Fac Pharmaceut Biomed & Vet Sci, Lab Microbiol Parasitol & Hyg, Antwerp, Belgium |
推荐引用方式 GB/T 7714 | Amin, Adnan,Tuenter, Emmy,Foubert, Kenn,et al. In Vitro and In Silico Antidiabetic and Antimicrobial Evaluation of Constituents from Kickxia ramosissima (Nanorrhinum ramosissimum)[J],2017,8. |
APA | Amin, Adnan.,Tuenter, Emmy.,Foubert, Kenn.,Iqbal, Jamhsed.,Cos, Paul.,...&Pieters, Luc.(2017).In Vitro and In Silico Antidiabetic and Antimicrobial Evaluation of Constituents from Kickxia ramosissima (Nanorrhinum ramosissimum).FRONTIERS IN PHARMACOLOGY,8. |
MLA | Amin, Adnan,et al."In Vitro and In Silico Antidiabetic and Antimicrobial Evaluation of Constituents from Kickxia ramosissima (Nanorrhinum ramosissimum)".FRONTIERS IN PHARMACOLOGY 8(2017). |
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