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DOI	10.1016/j.foodchem.2016.08.036
	Determination of irradiation histories of raw beef livers using liquid chromatography-tandem mass spectrometry of 5,6-dihydrothymidine
	Fukui, Naoki 1,2; Takatori, Satoshi 1; Kitagawa, Yoko 1; Okihashi, Masahiro 1; Ishikawa, Etsuko 2; Fujiyama, Takatomo 2; Kajimura, Keiji 1; Furuta, Masakazu 2; Obana, Hirotaka 1
通讯作者	Takatori, Satoshi
来源期刊	FOOD CHEMISTRY
ISSN	0308-8146
EISSN	1873-7072
出版年	2017
卷号	216 页码:186-193
英文摘要	A method for detecting irradiation histories of raw beef livers was developed by measuring 5,6-dihydrothymidine (DHdThd) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Liver DNA was extracted using phenol-chloroform extraction followed by precipitation in 50% ethanol. DNA was then enzymatically digested and nucleosides were purified using an OASIS MCX column. DHdThd and thymidine (dThd) contents of resulting test solutions were analyzed using LC-MS/MS. DHdThd was detected specifically after gamma-irradiation. Concentration ratios of DHdThd to dThd in the test solutions increased dose-dependently after irradiation at 1.0-11.3 kGy, which included the practical dose for sterilization of 2-7 kGy. Dose-response curves from beef livers of individual animals almost overlapped. Thus, this method is a candidate for the detection of irradiation histories of foods from which DNA can be extracted. (C) 2016 Elsevier Ltd. All rights reserved.
英文关键词	Irradiated food DNA 5,6-Dihydrothymidine Beef livers LC-MS/MS
类型	Article
语种	英语
国家	Japan
收录类别	SCI-E
WOS记录号	WOS:000384783300024
WOS关键词	ESCHERICHIA-COLI O111 ; CELLULAR-DNA ; COMET ASSAY ; AQUEOUS-SOLUTIONS ; BASE DAMAGE ; PRODUCTS ; RADIATION ; THYMIDINE ; FOOD ; IDENTIFICATION
WOS类目	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/198958
作者单位	1.Osaka Prefectural Inst Publ Hlth, Div Food Chem, Higashinari Ku, Nakamichi 1-3-69, Osaka, Japan; 2.Osaka Prefecture Univ, Ctr Radiat Res, Lab Quantum Beam Chem & Biol, 1-2 Gakuen Cho, Sakai, Osaka, Japan
推荐引用方式 GB/T 7714	Fukui, Naoki,Takatori, Satoshi,Kitagawa, Yoko,et al. Determination of irradiation histories of raw beef livers using liquid chromatography-tandem mass spectrometry of 5,6-dihydrothymidine[J],2017,216:186-193.
APA	Fukui, Naoki.,Takatori, Satoshi.,Kitagawa, Yoko.,Okihashi, Masahiro.,Ishikawa, Etsuko.,...&Obana, Hirotaka.(2017).Determination of irradiation histories of raw beef livers using liquid chromatography-tandem mass spectrometry of 5,6-dihydrothymidine.FOOD CHEMISTRY,216,186-193.
MLA	Fukui, Naoki,et al."Determination of irradiation histories of raw beef livers using liquid chromatography-tandem mass spectrometry of 5,6-dihydrothymidine".FOOD CHEMISTRY 216(2017):186-193.