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A simple and rapid method was developed for analysis of patulin in juice using ultra performance liquid chromatography coupled with tandem mass spectrometry. The samples were purified by an Oasis MAX 96-Well Plate. Chromatographic separation was performed on an ACQUITY UPLCA (R) HSS T3 column with gradient elution using acetonitrile and water as mobile phase. Analytes was quantified by external calibration curves over the ranges of 0.50-50 mu g/L, with correlation coefficients > 0.9986. The LOD and LOQ in juice sample were 0.30 and 1.0 mu g/L, respectively. Recoveries of the method (spiked at levels of 1, 5, 25 and 50 mu g/L) ranged from 91.6% to 95.7%, while intraday and interday relative standard deviations were 6.87-11.0% and 6.53-11.6%, respectively.