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If groundwater contains elevated levels of the fluoride ion (F), its consumption for drinking purposes is a major F exposure pathway for humans. Groundwater samples (n=100) were collected from 25 villages of District Mardan of the Khyber Pakhtunkhwa (KP) province, Pakistan, and analyzed for anions including F, chloride (Cl-), bromide (Br-), phosphate (PO43-), and sulfate (SO42-) with ion chromatography. The range of the F concentrations in the groundwater samples was 0.05-10.8 mg/L. The measured concentrations were within the permissible limits of National Environmental Quality Standards (NEQS) of Pakistan for drinking water in 92% of the samples. Groundwater samples of three villages exhibited F concentrations greater than the permissible level of 1.5 mg/L. The F concentration showed a positive correlation with electrical conductivity (EC), total dissolved solids (TDS), and the concentrations of Br-, PO43-, and SO42-. To safeguard public health, this study suggests the need for further research in the area for possible sources of F contamination and mitigation measures.