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In present investigation seroprevalence of trypanosomosis in buffaloes were assessed using monoclonal antibody based-latex agglutination test (TE-LAT). Out of 2494 buffaloes reported, a total of 890 serum samples of suspected buffaloes were collected during the period from June, 2013 to May, 2014, from various places of south western semi arid plane zone of Uttar Pradesh. Overall sseroprevalence of 18.56% and suspected seroprevalence of 52.02% were obtained indicating endemicity of surra in buffaloes of the region. Trypanosomosis was found prevalent throughout the year in the region and higher prevalence was observed in monsoon and post monsoon months followed by winter months and least in the summer months.