Arid
DOI10.1016/j.bbamcr.2017.06.003
Deep sequencing reveals a global reprogramming of lncRNA transcriptome during EMT
Liao, Jim-You1; Wu, Jue1,2; Wang, Yan-Jie1; He, Jie-Hua1; Deng, Wei-Xi1; Hu, KaiShun1; Zhang, Yu-Chan3,4; Zhang, Yin1; Yan, Haiyan1; Wang, Dan-Lan1; Liu, Qiang1; Zeng, Mu-Sheng5; Koeffler, H. Phillip6,7,8; Song, Erwei1; Yin, Dong1
通讯作者Yin, Dong
来源期刊BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
ISSN0167-4889
EISSN1879-2596
出版年2017
卷号1864期号:10页码:1703-1713
英文摘要

Several studies have shown that long non-coding RNAs (IncRNAs) may play an essential role in Epithelial-Mesenchymal Transition (EMT), which is an important step in tumor metastasis; however, little is known about the global change of IncRNA transcriptome during EMT. To investigate how IncRNA transcriptome alterations contribute to EMT progression regulation, we deep-sequenced the whole-transcriptome of MCF10A as the cells underwent TGF-beta-induced EMT.


Results: Deep-sequencing results showed that the long RNA transcriptome of MCF10A had undergone global changes as early as 8 h after treatment with TGF-beta. The expression of 3403 known and novel lncRNAs, and 570 known and novel circRNAs were altered during EMT. To identify the key lncRNA-regulator, we constructed the co-expression network and found all junction nodes in the network are lncRNAs. One junction node, RP6-65G23.5, was further verified as a key regulator of EMT. Intriguingly, we identified 216 clusters containing lncRNAs which were located in "gene desert" regions. The expressions of all lncRNAs in these clusters changed concurrently during EMT, strongly suggesting that these dusters might play important roles in EMT. Our study reveals a global reprogramming of lncRNAs transcriptome during EMT and provides clues for the future study of the molecular mechanism of EMT.


英文关键词IncRNA Transcriptome TGF-beta EMT circRNA
类型Article
语种英语
国家Peoples R China ; USA ; Singapore
收录类别SCI-E
WOS记录号WOS:000411168100016
WOS关键词LONG NONCODING RNAS ; SEQ ; ANNOTATION ; DATABASE ; CANCER ; CELLS ; GENE
WOS类目Biochemistry & Molecular Biology ; Cell Biology
WOS研究方向Biochemistry & Molecular Biology ; Cell Biology
来源机构University of California, Los Angeles
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/197783
作者单位1.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Res Ctr Med, Guangdong Prov Key Lab Malignant Tumor Epigenet &, Guangzhou 510120, Guangdong, Peoples R China;
2.Chinese Peoples Liberat Army Gen Hosp, Lab Translat Med, Beijing 100853, Peoples R China;
3.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China;
4.Sun Yat Sen Univ, Sch Life Sci, Key Lab Gene Engn, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China;
5.Sun Yat Sen Univ, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Guangdong, Peoples R China;
6.Univ Calif Los Angeles, Cedars Sinai Med Ctr, Div Hematol Oncol, Sch Med, Los Angeles, CA 90048 USA;
7.Natl Univ Singapore, Canc Sci Inst Singapore, Singapore, Singapore;
8.Natl Univ Singapore Hosp, Natl Univ Canc Inst, Singapore, Singapore
推荐引用方式
GB/T 7714
Liao, Jim-You,Wu, Jue,Wang, Yan-Jie,et al. Deep sequencing reveals a global reprogramming of lncRNA transcriptome during EMT[J]. University of California, Los Angeles,2017,1864(10):1703-1713.
APA Liao, Jim-You.,Wu, Jue.,Wang, Yan-Jie.,He, Jie-Hua.,Deng, Wei-Xi.,...&Yin, Dong.(2017).Deep sequencing reveals a global reprogramming of lncRNA transcriptome during EMT.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,1864(10),1703-1713.
MLA Liao, Jim-You,et al."Deep sequencing reveals a global reprogramming of lncRNA transcriptome during EMT".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1864.10(2017):1703-1713.
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