Arid
DOI10.1093/aob/mcw141
The Brassicaceae species Heliophila coronopifolia produces root border-like cells that protect the root tip and secrete defensin peptides
Weiller, Florent1,2; Moore, John P.1; Young, Philip1; Driouich, Azeddine2; Vivier, Melan A.1
通讯作者Moore, John P.
来源期刊ANNALS OF BOTANY
ISSN0305-7364
EISSN1095-8290
出版年2017
卷号119期号:5页码:803-813
英文摘要

Background and Aims Root border cells and border-like cells (BLCs), the latter originally described in Arabidopsis thaliana, have been described as cells released at the root tips of the species in which they occur. BLCs are thought to provide protection to root meristems similar to classical root border cells. In addition, four defensin peptides (Hc-AFP1-4) have previously been characterized from Heliophila coronopifolia, a South African semi-desert flower, and found to be strongly antifungal. This provided an opportunity to evaluate if the BLCs of H. coronopifolia indeed produce these defensins, which would provide evidence towards a defence role for BLCs.


Methods Fluorescence microscopy, using live-cell-imaging technology, was used to characterize the BLCs of H. coronopifolia. Quantitative real-time PCR (qRT-PCR) analysis and immunofluorescence microscopy was used to characterize these defensin peptides.


Key Results BLCs originated at the root apical meristem and formed a protective sheath at the tip and along the sides as the root elongated in solid medium. BLCs have a cellulose-enriched cell wall, intact nuclei and are embedded in a layer of pectin-rich mucilage. Pectinase treatments led to the dissolution of the sheath and dissociation of the root BLCs. Hc-AFP1-4 genes were all expressed in root tissues, but Hc-AFP3 transcripts were the most abundant in these tissues as measured by qRT-PCR. A polyclonal antibody that was cross-reactive with all four defensins, and probably recognizing a general plant defensin epitope, was used in fluorescence microscopy analysis to examine the presence of the peptides in the root tip and BLCs. Data confirmed the peptides present in the root tip tissues, the mucilage sheath and the BLCs.


Conclusions This study provides a link between defensin peptides and BLCs, both embedded in a protective pectin mucilage sheath, during normal plant growth and development. The presence of the Hc-AFP3 defensin peptides in the BLCs suggests a role for these cells in root protection.


英文关键词Brassicaceae Heliophila coronopifolia root border-like cells pectin-rich mucilage defensins pectinase Hc-AFP
类型Article
语种英语
国家South Africa ; France
收录类别SCI-E
WOS记录号WOS:000400982600009
WOS关键词ARABINOGALACTAN-PROTEINS ; ARABIDOPSIS ; EXPRESSION ; PEA ; GROWTH ; ORGANIZATION ; MORPHOLOGY ; PATTERN ; GENOME ; CAPS
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/197316
作者单位1.Stellenbosch Univ, Inst Wine Biotechnol, Dept Viticulture & Oenol, Fac AgriSci, ZA-7602 Matieland, South Africa;
2.Univ Rouen, Normandie Univ, Grand Reseau Rech VASI Haute Normandie, Lab Glycobiol & Matrice Extracellulaire Vegetale, F-76821 Mont St Aignan, France
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GB/T 7714
Weiller, Florent,Moore, John P.,Young, Philip,et al. The Brassicaceae species Heliophila coronopifolia produces root border-like cells that protect the root tip and secrete defensin peptides[J],2017,119(5):803-813.
APA Weiller, Florent,Moore, John P.,Young, Philip,Driouich, Azeddine,&Vivier, Melan A..(2017).The Brassicaceae species Heliophila coronopifolia produces root border-like cells that protect the root tip and secrete defensin peptides.ANNALS OF BOTANY,119(5),803-813.
MLA Weiller, Florent,et al."The Brassicaceae species Heliophila coronopifolia produces root border-like cells that protect the root tip and secrete defensin peptides".ANNALS OF BOTANY 119.5(2017):803-813.
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