Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s13205-017-0762-1 |
Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes | |
Sahay, Harmesh1,6; Yadav, Ajar Nath2; Singh, Atul Kumar3; Singh, Surendra1; Kaushik, Rajeev4; Saxena, Anil Kumar5 | |
通讯作者 | Saxena, Anil Kumar |
来源期刊 | 3 BIOTECH
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ISSN | 2190-572X |
EISSN | 2190-5738 |
出版年 | 2017 |
卷号 | 7 |
英文摘要 | Microbial communities in hot springs at high elevations have been extensively studied worldwide. In this sense, the Indian Himalaya regions is valuable ecosystems for providing both the extreme ’cold’ and ’hot’ sites for exploring microbial diversity. In the present study, a total of 140 thermophilic bacteria were isolated from 12 samples collected from Manikaran and Yumthang hot springs of Indian Himalayas. The bacterial isolates were studied for phylogenetic profiling, growth properties at varying conditions and potential sources of extracellular thermostable hydrolytic enzymes such as protease, amylase, xylanase and cellulase. Based on production of extracellular hydrolases, 51 isolates from Manikaran (28) and Yumthang thermal springs (23) were selected and identified using 16S rRNA gene sequencing which included 37 distinct species of 14 different genera namely Anoxybacillus, Bacillus, Brevibacillus, Brevundimonas, Burkholderia, Geobacillus, Paenibacillus, Planococcus, Pseudomonas, Rhodanobacter, Thermoactinomyces, Thermobacillus, Thermonema and Thiobacillus. Out of 51 hydrolase producing bacteria, 24 isolates showed stability at wide range of temperature and pH treatments. In present investigation, three thermotolerant bacteria namely, Thermobacillus sp NBM6, Paenibacillus ehimensis NBM24 and Paenibacillus popilliae NBM68 were found to produced cellulase-free xylanase. These potential extracellular thermostable hydrolytic enzymes producing thermophilic bacteria have a great commercial prospect in various industrial, medical and agriculture applications. |
英文关键词 | Thermostable extracellular hydrolases 16S rRNA gene Thermophiles Himalayan hot springs Diversity |
类型 | Article |
语种 | 英语 |
国家 | India ; USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000402725300011 |
WOS关键词 | PHYLOGENETIC DIVERSITY ; THERMOPHILIC BACTERIUM ; COLD DESERT ; SP NOV. ; BACILLI ; GEOBACILLUS ; CELLULOSE ; XYLANASE ; ODISHA |
WOS类目 | Biotechnology & Applied Microbiology |
WOS研究方向 | Biotechnology & Applied Microbiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/196924 |
作者单位 | 1.Rani Durgavati Univ, Dept Biol Sci, Jabalpur, India; 2.Eternal Univ, Dept Biotechnol, Akal Coll Agr, Baru Sahib, India; 3.Harvard Med Sch, Boston Childrens Hosp, Div Infect Dis, Boston, MA USA; 4.Indian Agr Res Inst, Div Microbiol, New Delhi, India; 5.Natl Bur Agr Important Microorganisms, Mau 275103, Uttar Pradesh, India; 6.R Biopharm Neugen Grp, Dept Res & Dev, Hyderabad, Andhra Pradesh, India |
推荐引用方式 GB/T 7714 | Sahay, Harmesh,Yadav, Ajar Nath,Singh, Atul Kumar,et al. Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes[J],2017,7. |
APA | Sahay, Harmesh,Yadav, Ajar Nath,Singh, Atul Kumar,Singh, Surendra,Kaushik, Rajeev,&Saxena, Anil Kumar.(2017).Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes.3 BIOTECH,7. |
MLA | Sahay, Harmesh,et al."Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes".3 BIOTECH 7(2017). |
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