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DOI10.1007/s13205-017-0762-1
Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes
Sahay, Harmesh1,6; Yadav, Ajar Nath2; Singh, Atul Kumar3; Singh, Surendra1; Kaushik, Rajeev4; Saxena, Anil Kumar5
通讯作者Saxena, Anil Kumar
来源期刊3 BIOTECH
ISSN2190-572X
EISSN2190-5738
出版年2017
卷号7
英文摘要

Microbial communities in hot springs at high elevations have been extensively studied worldwide. In this sense, the Indian Himalaya regions is valuable ecosystems for providing both the extreme ’cold’ and ’hot’ sites for exploring microbial diversity. In the present study, a total of 140 thermophilic bacteria were isolated from 12 samples collected from Manikaran and Yumthang hot springs of Indian Himalayas. The bacterial isolates were studied for phylogenetic profiling, growth properties at varying conditions and potential sources of extracellular thermostable hydrolytic enzymes such as protease, amylase, xylanase and cellulase. Based on production of extracellular hydrolases, 51 isolates from Manikaran (28) and Yumthang thermal springs (23) were selected and identified using 16S rRNA gene sequencing which included 37 distinct species of 14 different genera namely Anoxybacillus, Bacillus, Brevibacillus, Brevundimonas, Burkholderia, Geobacillus, Paenibacillus, Planococcus, Pseudomonas, Rhodanobacter, Thermoactinomyces, Thermobacillus, Thermonema and Thiobacillus. Out of 51 hydrolase producing bacteria, 24 isolates showed stability at wide range of temperature and pH treatments. In present investigation, three thermotolerant bacteria namely, Thermobacillus sp NBM6, Paenibacillus ehimensis NBM24 and Paenibacillus popilliae NBM68 were found to produced cellulase-free xylanase. These potential extracellular thermostable hydrolytic enzymes producing thermophilic bacteria have a great commercial prospect in various industrial, medical and agriculture applications.


英文关键词Thermostable extracellular hydrolases 16S rRNA gene Thermophiles Himalayan hot springs Diversity
类型Article
语种英语
国家India ; USA
收录类别SCI-E
WOS记录号WOS:000402725300011
WOS关键词PHYLOGENETIC DIVERSITY ; THERMOPHILIC BACTERIUM ; COLD DESERT ; SP NOV. ; BACILLI ; GEOBACILLUS ; CELLULOSE ; XYLANASE ; ODISHA
WOS类目Biotechnology & Applied Microbiology
WOS研究方向Biotechnology & Applied Microbiology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/196924
作者单位1.Rani Durgavati Univ, Dept Biol Sci, Jabalpur, India;
2.Eternal Univ, Dept Biotechnol, Akal Coll Agr, Baru Sahib, India;
3.Harvard Med Sch, Boston Childrens Hosp, Div Infect Dis, Boston, MA USA;
4.Indian Agr Res Inst, Div Microbiol, New Delhi, India;
5.Natl Bur Agr Important Microorganisms, Mau 275103, Uttar Pradesh, India;
6.R Biopharm Neugen Grp, Dept Res & Dev, Hyderabad, Andhra Pradesh, India
推荐引用方式
GB/T 7714
Sahay, Harmesh,Yadav, Ajar Nath,Singh, Atul Kumar,et al. Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes[J],2017,7.
APA Sahay, Harmesh,Yadav, Ajar Nath,Singh, Atul Kumar,Singh, Surendra,Kaushik, Rajeev,&Saxena, Anil Kumar.(2017).Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes.3 BIOTECH,7.
MLA Sahay, Harmesh,et al."Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes".3 BIOTECH 7(2017).
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