Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.talanta.2016.08.050 |
Online solid phase extraction and liquid chromatography-mass spectrometric determination of nucleoside drugs in plasma | |
Wang, Man1,2,3; Ma, Ping4; Xi, Xiaonan1,2,3; Liu, Lei1,2,3; Wen, Yabin5; Liu, Kangning1,2,3; Sun, Liang1,2,3; Lu, Yaxin1,2,3; Yin, Zheng1,2,3 | |
通讯作者 | Lu, Yaxin ; Yin, Zheng |
来源期刊 | TALANTA |
ISSN | 0039-9140 |
EISSN | 1873-3573 |
出版年 | 2016 |
卷号 | 161页码:278-287 |
英文摘要 | The bioanalysis and especially the sample preparation of nucleoside drugs in complex media, such as human plasma, has been challenging due to the high polarity and high solubility of these drugs in water. Online solid phase extraction (SPE) offers significant advantages, such as automation and timesaving. Thus, several types of SPE columns have been developed for compounds with different polarities. In this study, SPE was applied to overcome the issue of sample pretreatment of nucleoside drugs in human plasma, with the final aim of establishing a robust analytical platform for drugs with similar structures. A simple, easy-to-use, and efficient method is described for the simultaneous determination of lamivudine, zidovudine, didanosine and emtricitabine in human plasma via online SPE and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Following a simple centrifugation step, a 10 mu L plasma sample was injected directly onto the HPLC system. The Oasis MCX cartridge was washed, and the analytes were removed by back-flushing directly onto the analytical column. The analytes were quantified using a triple-quadrupole tandem mass spectrometer in multiple-reaction monitoring mode. Similarly, with the development and application of a Bond Elut phenylboronic acid (PBA) SPE cartridge, a fully automated online SPE-HPLC-MS/MS method was established for the simultaneous determination of ribavirin and taribavirin in human plasma. Linear calibration curves were obtained over the range of 0.5-2000 ng mL(-1), and the limit of quantification ranged from 0.5 ng mL(-1) to 10 ng mL(-1), which is sensitive enough for clinical drug monitoring. The intra- and inter-day precisions were in the range of 0.2-8.9%, and the trueness ranged between 88.9% and 113.1%. Excellent recoveries from plasma were achieved with a range between 86.7% and 105.1%. This procedure is easier to perform and requires less sample handling compared to methods previously described in the literature. This high-throughput method involving the direct injection of plasma samples may provide a practical solution for the analysis of multiple nucleoside drugs in clinical research. The method was tested in plasma samples from some patients and showed good performance. (C) 2016 Elsevier B.V. All rights reserved. |
英文关键词 | Nucleoside drugs Online SPE-LC-MS/MS Bioanalysis |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000386989500035 |
WOS关键词 | HYDROPHILIC INTERACTION CHROMATOGRAPHY ; HIV-INFECTED PATIENTS ; LC-MS/MS DETECTION ; RED-BLOOD-CELLS ; HUMAN SERUM ; LC/MS/MS METHOD ; HUMAN URINE ; RIBAVIRIN ; VALIDATION ; LAMIVUDINE |
WOS类目 | Chemistry, Analytical |
WOS研究方向 | Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/196625 |
作者单位 | 1.Nankai Univ, Coll Pharm, 94 Weijin Rd, Tianjin 300071, Peoples R China; 2.Nankai Univ, State Key Lab Elementoorgan Chem, 94 Weijin Rd, Tianjin 300071, Peoples R China; 3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China; 4.Tianjin Second Peoples Hosp, Dept Infect Dis, Tianjin 300071, Peoples R China; 5.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Man,Ma, Ping,Xi, Xiaonan,et al. Online solid phase extraction and liquid chromatography-mass spectrometric determination of nucleoside drugs in plasma[J],2016,161:278-287. |
APA | Wang, Man.,Ma, Ping.,Xi, Xiaonan.,Liu, Lei.,Wen, Yabin.,...&Yin, Zheng.(2016).Online solid phase extraction and liquid chromatography-mass spectrometric determination of nucleoside drugs in plasma.TALANTA,161,278-287. |
MLA | Wang, Man,et al."Online solid phase extraction and liquid chromatography-mass spectrometric determination of nucleoside drugs in plasma".TALANTA 161(2016):278-287. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。