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Tannase is an enzyme widely used in the food industry, mainly for wine and beer production. This enzyme has been identified in different fungus isolated from Mexican semi-desert plants. Some of these fungal strains such as Aspergillus niger GH1 and PSH strain have demonstrated to be excellent enzyme producers. In the present study, tannase was produced under submerged state culture, with its maximum activity at 24 h. At the same time, a fragment of tannase gene from Gill and PSH strains was isolated and expression of tannase was determinate by RT-PCR (at 16 h,). It was found that tannase sequences of Gill and PSH strain are 97% similar to the A. niger strain SL-5 reported in GeneBank. The translation of Gill and PSH amino acid sequences demonstrated its identity with tannase.