Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.4103/0973-1296.191455 |
Antihepatotoxic Effect and Metabolite Profiling of Panicum turgidum Extract via UPLC-qTOF-MS | |
Farag, Mohamed A.1; El Fishawy, Ahlam M.1; El-Toumy, Sayed A.2; Amer, Khadiga F.3; Mansour, Ahmed M.4; Taha, Hala E.3 | |
通讯作者 | Farag, Mohamed A. |
来源期刊 | PHARMACOGNOSY MAGAZINE |
ISSN | 0973-1296 |
EISSN | 0976-4062 |
出版年 | 2016 |
卷号 | 12期号:47页码:S446-S453 |
英文摘要 | Background: Panicum turgidum, desert grass, has not reported any detailed phytochemical or biological study as yet Objective: To establish P. turgidum secondary metabolite profile and to assess its antihepatotoxic effect Materials and Methods: Ultra-performance liquid chromatography (UPLC) coupled to quadrupole high-resolution time of flight mass spectrometry (qTOF-MS) was used for large-scale secondary metabolites profiling in P. turgidum extract, alongside assessing median lethal dose (LD50) and hepatoprotective effect against carbon tetrachloride (CCl4) intoxication Results: A total of 39 metabolites were identified with flavonoids as the major class present as O/C-glycosides of luteolin, apigenin, isorhamnetin and naringenin, most of which are first time to be reported in Panicum sp. Antihepatotoxic effect of P. turgidum crude extract was revealed via improving several biochemical marker levels and mitigation against oxidative stress in the serum and liver tissues, compared with CCl4 intoxicated group and further confirmed by histopathological examination. Conclusion: This study reveals that P. turgidum, enriched in C-flavonoids, presents a novel source of safe antihepatotoxic agents and further demonstrates the efficacy of UPLC-MS metabolomics in the field of natural products drug discovery. |
英文关键词 | Antihepatotoxic flavonoids Panicum turgidum toxicity UPLC MS analysis |
类型 | Article |
语种 | 英语 |
国家 | Egypt |
收录类别 | SCI-E |
WOS记录号 | WOS:000391129400010 |
WOS关键词 | MASS-SPECTROMETRY ; CLASSIFICATION |
WOS类目 | Chemistry, Medicinal |
WOS研究方向 | Pharmacology & Pharmacy |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/195430 |
作者单位 | 1.Cairo Univ, Dept Pharmacognosy, Coll Pharm, Cairo, Egypt; 2.Natl Res Ctr, Chem Tannins Dept, Cairo, Egypt; 3.Desert Res Ctr, Aromat & Med Plants Dept, Cairo, Egypt; 4.Al Azhar Univ, Dept Pharmacol & Toxicol, Fac Pharm, Cairo, Egypt |
推荐引用方式 GB/T 7714 | Farag, Mohamed A.,El Fishawy, Ahlam M.,El-Toumy, Sayed A.,et al. Antihepatotoxic Effect and Metabolite Profiling of Panicum turgidum Extract via UPLC-qTOF-MS[J],2016,12(47):S446-S453. |
APA | Farag, Mohamed A.,El Fishawy, Ahlam M.,El-Toumy, Sayed A.,Amer, Khadiga F.,Mansour, Ahmed M.,&Taha, Hala E..(2016).Antihepatotoxic Effect and Metabolite Profiling of Panicum turgidum Extract via UPLC-qTOF-MS.PHARMACOGNOSY MAGAZINE,12(47),S446-S453. |
MLA | Farag, Mohamed A.,et al."Antihepatotoxic Effect and Metabolite Profiling of Panicum turgidum Extract via UPLC-qTOF-MS".PHARMACOGNOSY MAGAZINE 12.47(2016):S446-S453. |
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