Arid
DOI10.1002/jsfa.7642
Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum
Castel, Virginia1; Zivanovic, Svetlana2; Jurat-Fuentes, Juan L.3; Santiago, Liliana G.1; Rubiolo, Amelia C.1; Carrara, Carlos R.1; Harted, Federico M.4
通讯作者Castel, Virginia
来源期刊JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
ISSN0022-5142
EISSN1097-0010
出版年2016
卷号96期号:13页码:4345-4350
英文摘要

BACKGROUND: Brea gum (BG) is an exudate from the Cercidium praecox tree that grows in semi-arid regions of Argentina. Some previous studies on BG have shown physicochemical characteristics and functional features similar to those of gum arabic. However, there is a need to elucidate the molecular structure of BG to understand the functionality. In this sense, BG was fractionated using hydrophobic interaction chromatography and the obtained fractions were analyzed by size exclusion chromatography.


RESULTS: Analysis of the fractions showed that the bulk of the gum (approx. 84% of the polysaccharides) was a polysaccharide of 2.79x10(3) kDa. The second major fraction (approx. 16% of the polysaccharides) was a polysaccharide-protein complex with a molecular mass of 1.92x10(5) kDa. A third fraction consisted of protein species with a wide range of molecular weights. The molecular weight distribution of the protein fraction was analyzed by size exclusion chromatography. Comparison of the elution profiles of the exudates in native and reducing conditions revealed that some of the proteins were forming aggregates through disulfide bridges in native conditions. Further analysis of the protein fraction by SDS-PAGE showed proteins with molecular weight ranging from 6.5 to 66 kDa.


CONCLUSIONS: The findings showed that BG consists of several fractions with heterogeneous chemical composition and polydisperse molecular weight distributions. (C) 2016 Society of Chemical Industry


英文关键词Cercidium praecox Brea gum plant gum exudate molecular mass distribution
类型Article
语种英语
国家Argentina ; USA
收录类别SCI-E
WOS记录号WOS:000382838100006
WOS关键词SENEGAL VAR. SENEGAL ; ACACIA-SENEGAL ; EXUDATE GUMS ; EMULSIONS ; PROTEIN ; WEIGHT ; TREES
WOS类目Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/194840
作者单位1.Univ Nacl Litoral, Fac Ingn Quim, Inst Tecnol Alimentos, Santa Fe, Santa Fe, Argentina;
2.Univ Tennessee, Dept Food Sci & Technol, Knoxville, TN 37996 USA;
3.Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA;
4.Penn State Univ, Dept Food Sci, University Pk, PA 16802 USA
推荐引用方式
GB/T 7714
Castel, Virginia,Zivanovic, Svetlana,Jurat-Fuentes, Juan L.,et al. Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum[J],2016,96(13):4345-4350.
APA Castel, Virginia.,Zivanovic, Svetlana.,Jurat-Fuentes, Juan L..,Santiago, Liliana G..,Rubiolo, Amelia C..,...&Harted, Federico M..(2016).Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum.JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,96(13),4345-4350.
MLA Castel, Virginia,et al."Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum".JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 96.13(2016):4345-4350.
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