Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1002/jsfa.7642 |
Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum | |
Castel, Virginia1; Zivanovic, Svetlana2; Jurat-Fuentes, Juan L.3; Santiago, Liliana G.1; Rubiolo, Amelia C.1; Carrara, Carlos R.1; Harted, Federico M.4 | |
通讯作者 | Castel, Virginia |
来源期刊 | JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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ISSN | 0022-5142 |
EISSN | 1097-0010 |
出版年 | 2016 |
卷号 | 96期号:13页码:4345-4350 |
英文摘要 | BACKGROUND: Brea gum (BG) is an exudate from the Cercidium praecox tree that grows in semi-arid regions of Argentina. Some previous studies on BG have shown physicochemical characteristics and functional features similar to those of gum arabic. However, there is a need to elucidate the molecular structure of BG to understand the functionality. In this sense, BG was fractionated using hydrophobic interaction chromatography and the obtained fractions were analyzed by size exclusion chromatography. RESULTS: Analysis of the fractions showed that the bulk of the gum (approx. 84% of the polysaccharides) was a polysaccharide of 2.79x10(3) kDa. The second major fraction (approx. 16% of the polysaccharides) was a polysaccharide-protein complex with a molecular mass of 1.92x10(5) kDa. A third fraction consisted of protein species with a wide range of molecular weights. The molecular weight distribution of the protein fraction was analyzed by size exclusion chromatography. Comparison of the elution profiles of the exudates in native and reducing conditions revealed that some of the proteins were forming aggregates through disulfide bridges in native conditions. Further analysis of the protein fraction by SDS-PAGE showed proteins with molecular weight ranging from 6.5 to 66 kDa. CONCLUSIONS: The findings showed that BG consists of several fractions with heterogeneous chemical composition and polydisperse molecular weight distributions. (C) 2016 Society of Chemical Industry |
英文关键词 | Cercidium praecox Brea gum plant gum exudate molecular mass distribution |
类型 | Article |
语种 | 英语 |
国家 | Argentina ; USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000382838100006 |
WOS关键词 | SENEGAL VAR. SENEGAL ; ACACIA-SENEGAL ; EXUDATE GUMS ; EMULSIONS ; PROTEIN ; WEIGHT ; TREES |
WOS类目 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/194840 |
作者单位 | 1.Univ Nacl Litoral, Fac Ingn Quim, Inst Tecnol Alimentos, Santa Fe, Santa Fe, Argentina; 2.Univ Tennessee, Dept Food Sci & Technol, Knoxville, TN 37996 USA; 3.Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA; 4.Penn State Univ, Dept Food Sci, University Pk, PA 16802 USA |
推荐引用方式 GB/T 7714 | Castel, Virginia,Zivanovic, Svetlana,Jurat-Fuentes, Juan L.,et al. Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum[J],2016,96(13):4345-4350. |
APA | Castel, Virginia.,Zivanovic, Svetlana.,Jurat-Fuentes, Juan L..,Santiago, Liliana G..,Rubiolo, Amelia C..,...&Harted, Federico M..(2016).Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum.JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,96(13),4345-4350. |
MLA | Castel, Virginia,et al."Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum".JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 96.13(2016):4345-4350. |
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