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A novel Gram-positive, oval-shaped, non-motile bacterium designated strain 16F1L(T) was isolated from sediment collected from the Han River in Seoul, Republic of Korea. Based on the 16S rRNA gene sequence (1,448 bp), this strain was identified as a member of the genus Deinococcus that belongs to the class Deinococci. Similarities in the 16S rRNA gene sequence were shown with Deinococcus daejeonensis MJ27(T) (99.0%), D. grandis DSM 3963(T) (98.1%), D. radiotolerans C1(T) (97.5%), and D. caeni Ho-08(T) (97.2%). Strain 16F1L(T) was classified as a different genomic species from closely related Deinococcus members, based on less than 70% DNA-DNA relatedness. Genomic DNA G+C content of strain 16F1L(T) was 67.2 mol%. Strain 16F1L(T) was found to grow at temperatures of 10-37A degrees C (optimum 25A degrees C) and pH 7-8 (optimum pH 7) on R2A medium, and was catalase-positive and oxidase-negative. Strain 16F1L(T) showed resistance to gamma radiation (D-10 > 2 kGy). In addition, this strain had the following chemotaxonomic characteristics: the major fatty acids were C-15:1 omega 6c and C-16:1 omega 7c; the polar lipid profile contained phosphoglycolipids, unknown aminophospholipids, an unknown aminoglycolipid, unknown aminolipids, an unknown glycolipid, an unknown phospholipid, and an unknown polar lipid; the major quinone was MK-8. Phylogenetic, genotypic, phenotypic, and chemotaxonomic characteristics indicated that strain 16F1L(T) represents a novel species within the genus Deinococcus, for which the name Deinococcus sedimenti sp. nov. is proposed. The type strain is 16F1L(T) (=KCTC 33796(T) =JCM 31405(T)).