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L-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates
Barati, Mahmood1; Faramarzi, Mohammad Ali2,3; Nafissi-Varcheh, Nastaran1; Khoshayand, Mohammad Reza4; Tehrani, Mohammad Hassan Houshdar5; Vahidi, Hossein1; Adrangi, Sina1
通讯作者Adrangi, Sina
来源期刊IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
ISSN1735-0328
EISSN1726-6890
出版年2016
卷号15期号:3页码:435-440
英文摘要

The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to grow in the selection medium containing 3.5 M NaCl. None of the isolates appeared to produce appreciable amounts of extracellular L-asparaginase. Among the isolates that produced intracellular L-asparaginase, 5 moderate and 1 extreme halophiles were selected for further study based on their observed activity level. The moderately halophilic isolates were shown to belong to the genus Halomonas while the extreme halophile was identified as a member of the genus Aidingimonas.


英文关键词Screening Halophile L-asparaginase Halomonas Aidingimonas
类型Article
语种英语
国家Iran
收录类别SCI-E
WOS记录号WOS:000391797700020
WOS关键词ACRYLAMIDE FORMATION ; HALOMONAS-ELONGATA ; MAILLARD REACTION ; RISK-ASSESSMENT ; PURIFICATION ; MICROORGANISMS ; MITIGATION ; TOLERANT ; STRAIN
WOS类目Pharmacology & Pharmacy
WOS研究方向Pharmacology & Pharmacy
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/193843
作者单位1.Shahid Beheshti Univ Med Sci, Sch Pharm, Dept Pharmaceut Biotechnol, Tehran, Iran;
2.Univ Tehran Med Sci, Fac Pharm, Dept Pharmaceut Biotechnol, POB 14155-6451, Tehran 14176, Iran;
3.Univ Tehran Med Sci, Biotechnol Res Ctr, POB 14155-6451, Tehran 14176, Iran;
4.Univ Tehran Med Sci, Dept Drug & Food Control, Fac Pharm, Tehran, Iran;
5.Shahid Beheshti Univ Med Sci, Dept Med Chem, Sch Pharm, Tehran, Iran
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GB/T 7714
Barati, Mahmood,Faramarzi, Mohammad Ali,Nafissi-Varcheh, Nastaran,et al. L-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates[J],2016,15(3):435-440.
APA Barati, Mahmood.,Faramarzi, Mohammad Ali.,Nafissi-Varcheh, Nastaran.,Khoshayand, Mohammad Reza.,Tehrani, Mohammad Hassan Houshdar.,...&Adrangi, Sina.(2016).L-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates.IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH,15(3),435-440.
MLA Barati, Mahmood,et al."L-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates".IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH 15.3(2016):435-440.
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