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A Gram-stain-positive, strictly aerobic strain, H6(T), was isolated from a soil sample of lead-cadmium tailing in Qixia district, Nanjing (China). Cells of the strain are rod-shaped and colonies on LB agar are red. Strain H6(T) has subpolar and polar flagella and the optimal condition for growth is 30 degrees C, with 1% (w/v) NaCl and at pH 7.0. Based on the 16S rRNA gene sequences, phylogenetic analysis showed that strain H6T was closely related to the genus Saccharibacillus, and the closest relatives were Saccharibacillus deserti WLJ055(T) (99.0% 16S rRNA gene sequence similarity), Saccharibacillus kuerlensis HR1(T) (97.0%) and Saccharibacillus sacchari GR21(T) (96.4 %). The DNA-DNA relatedness value between strain H6(T) and S. deserti WLJ055(T) was 55.0 %. The major polar lipids of strain H6(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid and three unknown glycolipids. The DNA G+C content was 58.4 mol% and MK-7 was the major isoprenoid quinone. The major fatty acids were anteiso-C-15 : 0 and C-16 : 0. meso-Diaminopimelic acid was detected in the peptidoglycan. Based on the phylogenetic, biochemical and chemotaxonomic data, strain H6(T) represents a novel species of the genus Saccharibacillus, for which the name Saccharibacillus qingshengii sp. nov., is proposed. The type strain is H6(T) (=CCTCC AB 2016001(T) = JCM 31172(T)).