Arid
DOI10.1111/imb.12222
The involvement of clathrin-mediated endocytosis and two Sid-1-like transmembrane proteins in doublestranded RNA uptake in the Colorado potato beetle midgut
Cappelle, K.1; de Oliveira, C. F. R.2; Van Eynde, B.1; Christiaens, O.1; Smagghe, G.1
通讯作者Cappelle, K. ; Smagghe, G.
来源期刊INSECT MOLECULAR BIOLOGY
ISSN0962-1075
EISSN1365-2583
出版年2016
卷号25期号:3页码:315-323
英文摘要

RNA interference (RNAi) is a powerful tool in entomology and shows promise as a crop protection strategy, but variability in its efficiency across different insect species limits its applicability. For oral uptake of the double-stranded RNA (dsRNA), the RNAi trigger, two different mechanisms are known: systemic RNA interference deficient-1 (Sid-1) transmembrane channel-mediated uptake and clathrin-mediated endocytosis. So far, a wide range of experiments has been conducted, confirming the involvement of one of the pathways in dsRNA uptake, but never both pathways in the same species. We investigated the role of both pathways in dsRNA uptake in the Colorado potato beetle, Leptinotarsa decemlineata, known to have an efficient RNAi response. Through RNAi-of-RNAi experiments, we demonstrated the contribution of two different sid-1-like (sil) genes, silA and silC, and clathrin heavy chain and the 16kDa subunit of the vacuolar H 1 ATPase (vha16), elements of the endocytic pathway, to the RNAi response. Furthermore, the sid-1-like genes were examined through phylogenetic and hydrophobicity analysis. This article reports for the first time on the involvement of two pathways in dsRNA uptake in an insect species and stresses the importance of evaluating both pathways through a well-devised reporter system in any future experiments on cellular dsRNA uptake.


英文关键词Colorado potato beetle RNA interference double-stranded RNA uptake systemic RNA interference deficient-1 (sid-1) endocytosis
类型Article
语种英语
国家Belgium ; Brazil
收录类别SCI-E
WOS记录号WOS:000375669800011
WOS关键词DOUBLE-STRANDED-RNA ; LEPTINOTARSA-DECEMLINEATA SAY ; SYSTEMIC RNAI ; SCHISTOCERCA-GREGARIA ; DESERT LOCUST ; INTERFERENCE ; GENES ; ELEGANS ; DROSOPHILA ; INSECT
WOS类目Biochemistry & Molecular Biology ; Entomology
WOS研究方向Biochemistry & Molecular Biology ; Entomology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/193616
作者单位1.Univ Ghent, Fac Biosci Engn, Dept Crop Protect, Coupure Links 653, B-9000 Ghent, Belgium;
2.Univ Estadual Campinas, Inst Biol, Dept Biochem, Campinas, SP, Brazil
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Cappelle, K.,de Oliveira, C. F. R.,Van Eynde, B.,et al. The involvement of clathrin-mediated endocytosis and two Sid-1-like transmembrane proteins in doublestranded RNA uptake in the Colorado potato beetle midgut[J],2016,25(3):315-323.
APA Cappelle, K.,de Oliveira, C. F. R.,Van Eynde, B.,Christiaens, O.,&Smagghe, G..(2016).The involvement of clathrin-mediated endocytosis and two Sid-1-like transmembrane proteins in doublestranded RNA uptake in the Colorado potato beetle midgut.INSECT MOLECULAR BIOLOGY,25(3),315-323.
MLA Cappelle, K.,et al."The involvement of clathrin-mediated endocytosis and two Sid-1-like transmembrane proteins in doublestranded RNA uptake in the Colorado potato beetle midgut".INSECT MOLECULAR BIOLOGY 25.3(2016):315-323.
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