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Reliable estimation of erosion in karst areas is difficult because of the heterogeneous nature of infiltration and subsurface drainage. Understanding the processes involved is a key requirement for managing against karst rock desertification. This study used the revised Universal Soil Loss Equation (RUSLE) to estimate the annual soil erosion rates on hillslopes and compared them with Cs-137 budget in the depressions at two typical karst peak-cluster depression basins in northwest Guangxi, southwestern China. Runoff plots data were used to calibrate the slope length factor (L) of the RUSLE model by adjusting the accumulated area threshold. The RUSLE model was sensitive to the value of the threshold and required DEMs with 1 m resolution, due to the discontinuous nature of the overland flow. The average annual soil erosion rates on hillslopes simulated by the RUSLE were 0.22 and 0.10 Mg ha(-1) y(-1) 2006 through 2011 in the partially cultivated GZ1 and the undisturbed GZ2 basins, respectively. The corresponding deposition rates in the depressions agreed well with the Cs-137 records when recent changes in precipitation and land use were taken into consideration. The study suggests that attention should be given to the RUSLE-L factor when applying the RUSLE on karst hillslopes because of the discontinuous nature of runoff and significant underground seepage during storm events that effectively reduces the effects of slope length. (C) 2015 Elsevier B.V. All rights reserved.