Arid
DOI10.1016/j.ygcen.2016.06.005
Molecular characterization of a short neuropeptide F signaling system in the tsetse fly, Glossina morsitans morsitans
Caers, Jelle1; Peymen, Katleen1; Van Hiel, Matthias B.1; Van Rompay, Liesbeth1; Van Den Abbeele, Jan2,3; Schoofs, Liliane1; Beets, Isabel1
通讯作者Schoofs, Liliane
来源期刊GENERAL AND COMPARATIVE ENDOCRINOLOGY
ISSN0016-6480
EISSN1095-6840
出版年2016
卷号235页码:142-149
英文摘要

Neuropeptides of the short neuropeptide F (sNPF) family are widespread among arthropods and found in every sequenced insect genome so far. Functional studies have mainly focused on the regulatory role of sNPF in feeding behavior, although this neuropeptide family has pleiotropic effects including in the control of locomotion, osmotic homeostasis, sleep, learning and memory. Here, we set out to characterize and determine possible roles of sNPF signaling in the haematophagous tsetse fly Glossina morsitans morsitans, a vector of African Trypanosome parasites causing human and animal African trypanosomiasis. We cloned the G. m. morsitans cDNA sequences of an sNPF-like receptor (Glomo-sNPFR) and precursor protein encoding four Glomo-sNPF neuropeptides. All four Glomo-sNPF peptides concentration-dependently activated Glomo-sNPFR in a cell-based calcium mobilization assay, with EC50) values in the nanomolar range. Gene expression profiles in adult female tsetse flies indicate that the Glomo-sNPF system is mainly restricted to the nervous system. Glomo-snpfr transcripts were also detected in the hindgut of adult females. In contrast to the Drosophila sNPF system, tsetse larvae lack expression of Glomo-snpf and Glomo-snpfr genes. While Glomo-snpf transcript levels are upregulated in pupae, the onset of Glomo-snpfr expression is delayed to adulthood. Expression profiles in adult tissues are similar to those in other insects suggesting that the tsetse sNPF system may have similar functions such as a regulatory role in feeding behavior, together with a possible involvement of sNPFR signaling in osmotic homeostasis. Our molecular data will enable further investigations into the functions of sNPF signaling in tsetse flies. (C) 2016 Published by Elsevier Inc.


英文关键词Insect Tsetse fly Glossina morsitans morsitans Neuropeptide Short neuropeptide F G protein-coupled receptor
类型Article
语种英语
国家Belgium
收录类别SCI-E
WOS记录号WOS:000382181800017
WOS关键词TIME QUANTITATIVE PCR ; DROSOPHILA-MELANOGASTER ; DESERT LOCUST ; GENE-EXPRESSION ; CENTRAL COMPLEX ; FOOD-INTAKE ; RECEPTOR ; PREDICTION ; NEURONS ; SITES
WOS类目Endocrinology & Metabolism
WOS研究方向Endocrinology & Metabolism
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/193129
作者单位1.Katholieke Univ Leuven, Dept Biol, Funct Genom & Prote, Naamsestr 59, B-3000 Leuven, Belgium;
2.Inst Trop Med, Dept Biomed Sci, Unit Vet Protozool, Antwerp, Belgium;
3.Univ Ghent, Dept Physiol, Lab Zoophysiol, Krijgslaan 281, B-9000 Ghent, Belgium
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GB/T 7714
Caers, Jelle,Peymen, Katleen,Van Hiel, Matthias B.,et al. Molecular characterization of a short neuropeptide F signaling system in the tsetse fly, Glossina morsitans morsitans[J],2016,235:142-149.
APA Caers, Jelle.,Peymen, Katleen.,Van Hiel, Matthias B..,Van Rompay, Liesbeth.,Van Den Abbeele, Jan.,...&Beets, Isabel.(2016).Molecular characterization of a short neuropeptide F signaling system in the tsetse fly, Glossina morsitans morsitans.GENERAL AND COMPARATIVE ENDOCRINOLOGY,235,142-149.
MLA Caers, Jelle,et al."Molecular characterization of a short neuropeptide F signaling system in the tsetse fly, Glossina morsitans morsitans".GENERAL AND COMPARATIVE ENDOCRINOLOGY 235(2016):142-149.
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