Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.gene.2016.03.039 |
Cloning and characterization of metallothionein gene (HcMT) from Halostachys caspica and its expression in E. coli | |
Liu, Zhongyuan; Meng, Hongen; Abdulla, Hasiyatihan; Zhang, Fuchun; Mao, Xinfang | |
通讯作者 | Mao, Xinfang |
来源期刊 | GENE |
ISSN | 0378-1119 |
EISSN | 1879-0038 |
出版年 | 2016 |
卷号 | 585期号:2页码:221-227 |
英文摘要 | Halostachys caspica is a short shrub distributed in the semi-arid and saline-alkali area, which evolved various mechanisms for modulating salt and metal level. In the present study, a Type 2 metallothionein (HcMT) gene was cloned from the salt induced suppression subtractive hybridization (SSH) cDNA library of H.caspica. Quantitative real time PCR (qRT-PCR) analysis indicated that HcMT gene was up-regulated under the stress of Cu2+, Zn2+ and Cd2+, and the tolerance of E. coli strain harboring with the recombinant HcMT (pET-32a-HcMT) to Cu2+, Zn2+ and Cd2+ was enhanced compared to strain with control vector (pET-32a). Moreover, the purified TrxA-HcMT fusion protein from E. coli cells grown in the presence of 0.3 mM CuSO4, 03 mM ZnSO4, or 0.1 mM CdCl2 could bind more metal ions than TrxA alone. The predicted 3D structure showed that HcMT could form a single metal-thiolate cluster, which confers the ability to bind five divalent metal ions through fourteen cysteine residues. These data indicate that HcMT may be involved in processes of metal tolerance in H. caspica and could be employed as a potential candidate for heavy metal phytoremediation. (C) 2016 Elsevier B.V. All rights reserved. |
英文关键词 | Metallothionein Halostachys caspica Metal tolerance Metal binding Phytoremediation |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000375519500006 |
WOS关键词 | TYPE-2 METALLOTHIONEIN ; HETEROLOGOUS EXPRESSION ; STRESS |
WOS类目 | Genetics & Heredity |
WOS研究方向 | Genetics & Heredity |
来源机构 | 新疆大学 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/193124 |
作者单位 | Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, 14 Shengli Rd, Urumqi 830046, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Zhongyuan,Meng, Hongen,Abdulla, Hasiyatihan,et al. Cloning and characterization of metallothionein gene (HcMT) from Halostachys caspica and its expression in E. coli[J]. 新疆大学,2016,585(2):221-227. |
APA | Liu, Zhongyuan,Meng, Hongen,Abdulla, Hasiyatihan,Zhang, Fuchun,&Mao, Xinfang.(2016).Cloning and characterization of metallothionein gene (HcMT) from Halostachys caspica and its expression in E. coli.GENE,585(2),221-227. |
MLA | Liu, Zhongyuan,et al."Cloning and characterization of metallothionein gene (HcMT) from Halostachys caspica and its expression in E. coli".GENE 585.2(2016):221-227. |
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