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Present study has analysed stable isotopic composition (delta O-18 and delta H-2) of cryospheric waters of Ladakh and Kashmir regions, located in Western Himalayas, India. The samples of glacier ice (11) and melt water stream (10) collected altitude ranging between 3168 and 5465 m a.s.l. have been analysed for delta O-18 and delta H-2. The measured delta O-18 values of glacier ice varied between -16.5 and -13.8 parts per thousand in Ladakh, and between -12.8 and -9.6 parts per thousand in Kashmir region. Study shows that isotopic characteristics of ice samples collected from snout of glaciers in Ladakh and Kashmir region are distinctly different from each other. Melt water samples collected from streams near the snout during September have shown good resemblance with the glacier ice melt of respective region. The d-excess of glaciers and melt water of Kashmir region varied from 21.0 to 25.6 parts per thousand and from 18.0 to 21.5 parts per thousand respectively. The d-excess of glaciers of Ladakh region have been found between 13.5 and 16.7 parts per thousand and of the melt water between 11.6 and 12.6 parts per thousand. Depleted delta O-18 signature and low delta-excess value of glaciers of Ladakh in comparison with that of Kashmir region may be attributed to sublimation process triggered by cold arid climatic conditions. The present study of Kashmir and Ladakh region suggest that isotopic composition of glacier ice is influenced by post depositional alteration, such as sublimation, during snow to glacier ice transformation.