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Oil-source rock correlations are used in this study in order to achieve their relationship, depositional environments, and diagenetic processes in the source rocks. For this purpose many parameters are used, e.g., gross composition of oil and source rock extracts, and biomarker analyses. Ten representative oil samples and seven source rock hydrocarbon extracts were analyzed using relative geochemical analyses, and gas chromatography-mass spectrometry. The results revealed a genetic close relation between them and supported the indigenous mixed source of Alam El Bueib oil, which related to different sources, including Khatatba, Alam El Bueib, and Bahariya formations. Accordingly, Alam El Bueib formation can be considered as an important source for petroleum generation in that area.