Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.cryobiol.2016.08.012 |
Pretreatment of Addra gazelle (Nanger dama ruficollis) spermatozoa with cholesterol-loaded cyclodextrins improves cryosurvival | |
Wojtusik, Jessye1,2,4; Pennington, Parker1,2,5; Songsasen, Nucharin1; Padilla, Luis R.1,6; Citino, Scott B.3; Pukazhenthi, Budhan S.1 | |
通讯作者 | Pukazhenthi, Budhan S. |
来源期刊 | CRYOBIOLOGY |
ISSN | 0011-2240 |
EISSN | 1090-2392 |
出版年 | 2016 |
卷号 | 73期号:3页码:388-395 |
英文摘要 | Preserving genetic diversity of the critically endangered Addra gazelle (Nanger dama ruficollis) could be enhanced through the use of frozen-thawed sperm and artificial insemination. Our aim was to characterize Addra ejaculate traits and to assess the effects of cholesterol-loaded cyclodextrin (CLC) on sperm cryosurvival. Fresh ejaculates were treated with CLC (0, 0.5, 1.5, 3.0, and 6.0 mg/ml) prior to cryopreservation. All males produced spermic ejaculates with >75% sperm motility. The mean +/- SEM seminal volume, sperm concentration, percent motility, forward progression, and percent morphologically normal spermatozoa were 3.2 +/- 0.3 ml, 1.2 03 x 10(9), 75.82 +/- 2.7%, 3.2 +/- 0.3 (0-5 scale; 5 = most progressive), and 57.12 +/- 3.8%, respectively. More than 92% contained an intact acrosome. There was no effect of time or in vitro incubation on progression or acrosomal integrity on thawed samples (P > 0.05). Spermatozoa pre-treated with 0.5 mg/ml CLC retained higher (P < 0.05) motility post-thaw than aliquots treated with 0, 3.0, or 6.0 mg/ml of CLC. Spermatozoa pre-treated with 0.5, 1.5, or 3.0 mg/ml CLC exhibited greater viability than counterparts (P < 0.05). Sperm kinetics including beat cross frequency (BCE), average path velocity (VAP), curvilinear velocity (VCL), and straight line velocity (VSL) did not differ among samples (P > 0.05). Linearity (LIN) and straightness (STR) were different among samples after thawing. Results demonstrate treatment with CLC (0.5 mg/ml) protects Addra spermatozoa from cryo-damage. Reported advances will facilitate establishment of a frozen repository and support the genetic management of this critically endangered north African desert antelope. Published by Elsevier Inc. |
英文关键词 | Cryopreservation Cholesterol Antelope Acrosome reaction Endangered species |
类型 | Article |
语种 | 英语 |
国家 | USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000389010600013 |
WOS关键词 | OSMOTIC TOLERANCE LIMITS ; METHYL-BETA-CYCLODEXTRIN ; GENETIC RESOURCE BANKS ; BOAR SPERMATOZOA ; PLASMA-MEMBRANE ; COLD-SHOCK ; BULL SPERM ; ARTIFICIAL-INSEMINATION ; SEMEN CRYOPRESERVATION ; FLUCTUATING ASYMMETRY |
WOS类目 | Biology ; Physiology |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Physiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/192200 |
作者单位 | 1.Smithsonian Conservat Biol Inst, Ctr Species Survival, 1500 Remount Rd, Front Royal, VA 22630 USA; 2.George Mason Univ, Dept Environm Sci & Policy, 4400 Univ Dr, Fairfax, VA 22030 USA; 3.White Oak Conservat, 581705 Gilman Dr, Yulee, FL 32097 USA; 4.Cincinnati Zoo & Bot Garden, Ctr Conservat & Res Endangered Wildlife CREW, 3400 Vine St, Cincinnati, OH 45220 USA; 5.San Diego Zoo Global, Inst Conservat Res, 15600 San Pasqual Valley Rd, Escondido, CA 92027 USA; 6.St Louis Zoo, 1 Govt Dr, St Louis, MO 63110 USA |
推荐引用方式 GB/T 7714 | Wojtusik, Jessye,Pennington, Parker,Songsasen, Nucharin,et al. Pretreatment of Addra gazelle (Nanger dama ruficollis) spermatozoa with cholesterol-loaded cyclodextrins improves cryosurvival[J],2016,73(3):388-395. |
APA | Wojtusik, Jessye,Pennington, Parker,Songsasen, Nucharin,Padilla, Luis R.,Citino, Scott B.,&Pukazhenthi, Budhan S..(2016).Pretreatment of Addra gazelle (Nanger dama ruficollis) spermatozoa with cholesterol-loaded cyclodextrins improves cryosurvival.CRYOBIOLOGY,73(3),388-395. |
MLA | Wojtusik, Jessye,et al."Pretreatment of Addra gazelle (Nanger dama ruficollis) spermatozoa with cholesterol-loaded cyclodextrins improves cryosurvival".CRYOBIOLOGY 73.3(2016):388-395. |
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