Arid
DOI10.1186/s12870-016-0938-6
Analysis of transcriptional response to heat stress in Rhazya stricta
Obaid, Abdullah Y.1; Sabir, Jamal S. M.1; Atef, Ahmed1; Liu, Xuan2; Edris, Sherif1,3,4; El-Domyati, Fotouh M.1,3; Mutwakil, Mohammed Z.1; Gadalla, Nour O.1,5; Hajrah, Nahid H.1; Al-Kordy, Magdy A.1,5; Hall, Neil1,2; Bahieldin, Ahmed1,3; Jansen, Robert K.1,6
通讯作者Jansen, Robert K.
来源期刊BMC PLANT BIOLOGY
ISSN1471-2229
出版年2016
卷号16
英文摘要

Background: Climate change is predicted to be a serious threat to agriculture due to the need for crops to be able to tolerate increased heat stress. Desert plants have already adapted to high levels of heat stress so they make excellent systems for identifying genes involved in thermotolerance. Rhazya stricta is an evergreen shrub that is native to extremely hot regions across Western and South Asia, making it an excellent system for examining plant responses to heat stress. Transcriptomes of apical and mature leaves of R. stricta were analyzed at different temperatures during several time points of the day to detect heat response mechanisms that might confer thermotolerance and protection of the plant photosynthetic apparatus.


Results: Biological pathways that were crosstalking during the day involved the biosynthesis of several heat stress-related compounds, including soluble sugars, polyols, secondary metabolites, phenolics and methionine. Highly downregulated leaf transcripts at the hottest time of the day (40-42.4 degrees C) included genes encoding cyclin, cytochrome p450/secologanin synthase and U-box containing proteins, while upregulated, abundant transcripts included genes encoding heat shock proteins (HSPs), chaperones, UDP-glycosyltransferase, aquaporins and protein transparent testa 12. The upregulation of transcripts encoding HSPs, chaperones and UDP-glucosyltransferase and downregulation of transcripts encoding U-box containing proteins likely contributed to thermotolerance in R. stricta leaf by correcting protein folding and preventing protein degradation. Transcription factors that may regulate expression of genes encoding HSPs and chaperones under heat stress included HSFA2 to 4, AP2-EREBP and WRKY27.


Conclusion: This study contributed new insights into the regulatory mechanisms of thermotolerance in the wild plant species R. stricta, an arid land, perennial evergreen shrub common in the Arabian Peninsula and Indian subcontinent. Enzymes from several pathways are interacting in the biosynthesis of soluble sugars, polyols, secondary metabolites, phenolics and methionine and are the primary contributors to thermotolerance in this species.


英文关键词Thermotolerance HSP Chaperones HSF Cyclin U-box Aquaporine Protein transparent testa 12 AP2-EREBP WRKY27
类型Article
语种英语
国家Saudi Arabia ; England ; Egypt ; USA
收录类别SCI-E
WOS记录号WOS:000389481300001
WOS关键词E3 UBIQUITIN LIGASES ; SHOCK-PROTEIN GENE ; HIGH-TEMPERATURE ; ABIOTIC STRESS ; STARCH METABOLISM ; DROUGHT STRESS ; MOLECULAR CHAPERONES ; RUBISCO ACTIVASE ; OXIDATIVE STRESS ; QUALITY-CONTROL
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/191861
作者单位1.King Abdulaziz Univ, Dept Biol Sci, Fac Sci, POB 80141, Jeddah 21589, Saudi Arabia;
2.Univ Liverpool, Inst Integrat Biol, Liverpool L69 7ZB, Merseyside, England;
3.Ain Shams Univ, Dept Genet, Fac Agr, Cairo, Egypt;
4.King Abdulaziz Univ, Princess Al Jawhara Al Brahim Ctr Excellence Res, Fac Med, Jeddah, Saudi Arabia;
5.Natl Res Ctr, Genet Engn & Biotechnol Div, Genet & Cytol Dept, Dokki, Egypt;
6.Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA
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GB/T 7714
Obaid, Abdullah Y.,Sabir, Jamal S. M.,Atef, Ahmed,et al. Analysis of transcriptional response to heat stress in Rhazya stricta[J],2016,16.
APA Obaid, Abdullah Y..,Sabir, Jamal S. M..,Atef, Ahmed.,Liu, Xuan.,Edris, Sherif.,...&Jansen, Robert K..(2016).Analysis of transcriptional response to heat stress in Rhazya stricta.BMC PLANT BIOLOGY,16.
MLA Obaid, Abdullah Y.,et al."Analysis of transcriptional response to heat stress in Rhazya stricta".BMC PLANT BIOLOGY 16(2016).
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