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DOI10.1002/bmc.3562
A robust and rapid liquid chromatography tandem mass spectrometric method for the quantitative analysis of 5-azacytidine
Anders, Nicole M.; Wanjiku, Teresia M.; He, Ping; Azad, Nilofer S.; Rudek, Michelle A.
通讯作者Rudek, Michelle A.
来源期刊BIOMEDICAL CHROMATOGRAPHY
ISSN0269-3879
EISSN1099-0801
出版年2016
卷号30期号:3页码:494-496
英文摘要

The DNA methyltransferase inhibitor 5-azacytidine is being evaluated clinically as an oral formulation to treat various solid tumors. A sensitive, reliable method was developed to quantitate 5-azacytidine using LC-MS/MS to perform detailed pharmacokinetic studies. The drug of interest was extracted from plasma using Oasis MCX ion exchange solid-phase extraction 96-well plates. Chromatographic separation was achieved with a YMC J’sphere M80 C-18 column and isocratic elution with a methanol-water-formic acid (15:85:0.1, v/v/v) mobile phase over a 7 min total analytical run time. An AB Sciex 5500 triple quadrupole mass spectrometer operated in positive electrospray ionization mode was used for the detection of 5-azacytidine. The assay range was 5-500 ng/mL and proved to be accurate (97.8-109.1%) and precise (CV 9.8%). Tetrahydrouridine was used to stabilize 5-azacytidine in blood/plasma samples. With the addition of tetrahydrouridine, long-term frozen plasma stability for 5-azacytidine at -70 degrees C has been determined for at least 323 days. The method was applied for the measurement of total plasma concentrations of 5-azacytidine in a cancer patient receiving a 300 mg oral daily dose. Copyright (c) 2015 John Wiley & Sons, Ltd.


英文关键词5-azacytidine DNA methyltransferase inhibitor LC MS MS pharmacokinetics
类型Article
语种英语
国家USA
收录类别SCI-E
WOS记录号WOS:000369735700025
WOS关键词HEMATOLOGIC MALIGNANCIES ; CYTIDINE DEAMINASE ; AZACITIDINE ; PHARMACOKINETICS ; DEGRADATION
WOS类目Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/191813
作者单位Johns Hopkins Univ, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD USA
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GB/T 7714
Anders, Nicole M.,Wanjiku, Teresia M.,He, Ping,et al. A robust and rapid liquid chromatography tandem mass spectrometric method for the quantitative analysis of 5-azacytidine[J],2016,30(3):494-496.
APA Anders, Nicole M.,Wanjiku, Teresia M.,He, Ping,Azad, Nilofer S.,&Rudek, Michelle A..(2016).A robust and rapid liquid chromatography tandem mass spectrometric method for the quantitative analysis of 5-azacytidine.BIOMEDICAL CHROMATOGRAPHY,30(3),494-496.
MLA Anders, Nicole M.,et al."A robust and rapid liquid chromatography tandem mass spectrometric method for the quantitative analysis of 5-azacytidine".BIOMEDICAL CHROMATOGRAPHY 30.3(2016):494-496.
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