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DOI | 10.1002/bmc.3562 |
A robust and rapid liquid chromatography tandem mass spectrometric method for the quantitative analysis of 5-azacytidine | |
Anders, Nicole M.; Wanjiku, Teresia M.; He, Ping; Azad, Nilofer S.; Rudek, Michelle A. | |
通讯作者 | Rudek, Michelle A. |
来源期刊 | BIOMEDICAL CHROMATOGRAPHY
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ISSN | 0269-3879 |
EISSN | 1099-0801 |
出版年 | 2016 |
卷号 | 30期号:3页码:494-496 |
英文摘要 | The DNA methyltransferase inhibitor 5-azacytidine is being evaluated clinically as an oral formulation to treat various solid tumors. A sensitive, reliable method was developed to quantitate 5-azacytidine using LC-MS/MS to perform detailed pharmacokinetic studies. The drug of interest was extracted from plasma using Oasis MCX ion exchange solid-phase extraction 96-well plates. Chromatographic separation was achieved with a YMC J’sphere M80 C-18 column and isocratic elution with a methanol-water-formic acid (15:85:0.1, v/v/v) mobile phase over a 7 min total analytical run time. An AB Sciex 5500 triple quadrupole mass spectrometer operated in positive electrospray ionization mode was used for the detection of 5-azacytidine. The assay range was 5-500 ng/mL and proved to be accurate (97.8-109.1%) and precise (CV 9.8%). Tetrahydrouridine was used to stabilize 5-azacytidine in blood/plasma samples. With the addition of tetrahydrouridine, long-term frozen plasma stability for 5-azacytidine at -70 degrees C has been determined for at least 323 days. The method was applied for the measurement of total plasma concentrations of 5-azacytidine in a cancer patient receiving a 300 mg oral daily dose. Copyright (c) 2015 John Wiley & Sons, Ltd. |
英文关键词 | 5-azacytidine DNA methyltransferase inhibitor LC MS MS pharmacokinetics |
类型 | Article |
语种 | 英语 |
国家 | USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000369735700025 |
WOS关键词 | HEMATOLOGIC MALIGNANCIES ; CYTIDINE DEAMINASE ; AZACITIDINE ; PHARMACOKINETICS ; DEGRADATION |
WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/191813 |
作者单位 | Johns Hopkins Univ, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD USA |
推荐引用方式 GB/T 7714 | Anders, Nicole M.,Wanjiku, Teresia M.,He, Ping,et al. A robust and rapid liquid chromatography tandem mass spectrometric method for the quantitative analysis of 5-azacytidine[J],2016,30(3):494-496. |
APA | Anders, Nicole M.,Wanjiku, Teresia M.,He, Ping,Azad, Nilofer S.,&Rudek, Michelle A..(2016).A robust and rapid liquid chromatography tandem mass spectrometric method for the quantitative analysis of 5-azacytidine.BIOMEDICAL CHROMATOGRAPHY,30(3),494-496. |
MLA | Anders, Nicole M.,et al."A robust and rapid liquid chromatography tandem mass spectrometric method for the quantitative analysis of 5-azacytidine".BIOMEDICAL CHROMATOGRAPHY 30.3(2016):494-496. |
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