Arid
DOI10.1007/s00374-016-1107-7
Use of quantitative PCR with the chloroplast gene rps4 to determine moss abundance in the early succession stage of biological soil crusts
Deng, Songqiang1; Wang, Chunzi1; De Philippis, Roberto2; Zhou, Xiangjun1; Ye, Chaoran1; Chen, Lanzhou1
通讯作者Chen, Lanzhou
来源期刊BIOLOGY AND FERTILITY OF SOILS
ISSN0178-2762
EISSN1432-0789
出版年2016
卷号52期号:5页码:595-599
英文摘要

The quantitative PCR (qPCR) method was firstly used to measure moss abundance with moss chloroplast gene rps4 as compared to that with cyanobacterial 16S rRNA gene and fungal 25-28S rRNA gene in the early succession stage of biological soil crusts (BSCs). Four sites with three BSC types collected from Hobq Desert of China, representing cyanobacterial-, lichen-, and moss-dominated BSCs were investigated. The copies of the moss rps4 gene, cyanobacterial 16S rRNA gene, and fungal 25-28S rRNA gene, chlorophyll a content, and the community composition variated significantly. The moss rps4 gene copies attained a significant positive correlation with chlorophyll a content and showed a cross-validation with relative moss biomass.


英文关键词Moss Quantitative PCR rps4 Biomass Community composition
类型Article
语种英语
国家Peoples R China ; Italy
收录类别SCI-E
WOS记录号WOS:000377994900001
WOS关键词MOJAVE DESERT ; BACTERIAL ; POLYSACCHARIDES ; COMMUNITIES ; DIVERSITY ; PRIMERS ; MODEL
WOS类目Soil Science
WOS研究方向Agriculture
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/191800
作者单位1.Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm B, Wuhan 430079, Peoples R China;
2.Univ Florence, Dept Agrifood Prod & Environm Sci, Piazzale Cascine 24, I-50144 Florence, Italy
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Deng, Songqiang,Wang, Chunzi,De Philippis, Roberto,et al. Use of quantitative PCR with the chloroplast gene rps4 to determine moss abundance in the early succession stage of biological soil crusts[J],2016,52(5):595-599.
APA Deng, Songqiang,Wang, Chunzi,De Philippis, Roberto,Zhou, Xiangjun,Ye, Chaoran,&Chen, Lanzhou.(2016).Use of quantitative PCR with the chloroplast gene rps4 to determine moss abundance in the early succession stage of biological soil crusts.BIOLOGY AND FERTILITY OF SOILS,52(5),595-599.
MLA Deng, Songqiang,et al."Use of quantitative PCR with the chloroplast gene rps4 to determine moss abundance in the early succession stage of biological soil crusts".BIOLOGY AND FERTILITY OF SOILS 52.5(2016):595-599.
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