Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1248/bpb.b15-00573 |
Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season | |
Yamaguchi, Nobuyasu1; Baba, Takashi1,2; Ichijo, Tomoaki1; Himezawa, Yuka3; Enoki, Kanami3; Saraya, Makoto1,4; Li, Pin-Fang5; Nasu, Masao1 | |
通讯作者 | Yamaguchi, Nobuyasu |
来源期刊 | BIOLOGICAL & PHARMACEUTICAL BULLETIN
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ISSN | 0918-6158 |
出版年 | 2016 |
卷号 | 39期号:1页码:68-77 |
英文摘要 | Approximately 180 t/km(2) of Asian dust particles are estimated to fall annually on Beijing, China, and there is significant concern about the influence of microbes transported by Asian dust events on human health and downwind ecosystems. In this study, we collected Asian dust particles in Beijing, and analyzed the bacterial communities on these particles by culture-independent methods. Bacterial cells on Asian dust particles were visualized first by laser scanning microscopy, which demonstrated that Asian dust particles carry bacterial cells to Beijing. Bacterial abundance, as determined by quantitative polymerase chain reaction (PCR), was 10(8) to 10(9)cells/g, a value about 10 times higher than that in Asian dust source soils. Inter-seasonal variability of bacterial community structures among Asian dust samples, as compared by terminal restriction fragment length polymorphism (T-RFLP), was low during the Asian dust season. Several viable bacteria, including intestinal bacteria, were found in Asian dust samples by denaturing gradient gel electrophoresis (DGGE). Clone library analysis targeting 16S ribosomal RNA (rRNA) gene sequences demonstrated that bacterial phylogenetic diversity was high in the dust samples, and most of these were environmental bacteria distributed in soil and air. The dominant species in the clone library was Segetibacter aerophilus (Bacteroidetes), which was first isolated from an Asian dust sample collected in Korea. Our results also indicate the possibility of a change in the bacterial community structure during transportation and increases in desiccation-tolerant bacteria such as Firmicutes. |
英文关键词 | Asian dust bacteria long-range transportation laser scanning microscopy 16S ribosomal RNA (rRNA) gene library phylogenetic analysis |
类型 | Article |
语种 | 英语 |
国家 | Japan ; Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000367359300008 |
WOS关键词 | FRAGMENT-LENGTH-POLYMORPHISM ; RIBOSOMAL-RNA ; DESERT-DUST ; MICROBIAL COMMUNITY ; SOIL DUST ; SP NOV. ; TRANSPORT ; ENVIRONMENTS ; DIVERSITY ; ARCHAEA |
WOS类目 | Pharmacology & Pharmacy |
WOS研究方向 | Pharmacology & Pharmacy |
来源机构 | 中国农业大学 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/191765 |
作者单位 | 1.Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan; 2.Tottori Univ, Fac Agr, Tottori 6808553, Japan; 3.Osaka Univ, Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan; 4.Himeji Dokkyo Univ, Fac Pharmaceut Sci, Himeji, Hyogo 6708524, Japan; 5.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China |
推荐引用方式 GB/T 7714 | Yamaguchi, Nobuyasu,Baba, Takashi,Ichijo, Tomoaki,et al. Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season[J]. 中国农业大学,2016,39(1):68-77. |
APA | Yamaguchi, Nobuyasu.,Baba, Takashi.,Ichijo, Tomoaki.,Himezawa, Yuka.,Enoki, Kanami.,...&Nasu, Masao.(2016).Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season.BIOLOGICAL & PHARMACEUTICAL BULLETIN,39(1),68-77. |
MLA | Yamaguchi, Nobuyasu,et al."Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season".BIOLOGICAL & PHARMACEUTICAL BULLETIN 39.1(2016):68-77. |
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