Arid
DOI10.1248/bpb.b15-00573
Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season
Yamaguchi, Nobuyasu1; Baba, Takashi1,2; Ichijo, Tomoaki1; Himezawa, Yuka3; Enoki, Kanami3; Saraya, Makoto1,4; Li, Pin-Fang5; Nasu, Masao1
通讯作者Yamaguchi, Nobuyasu
来源期刊BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN0918-6158
出版年2016
卷号39期号:1页码:68-77
英文摘要

Approximately 180 t/km(2) of Asian dust particles are estimated to fall annually on Beijing, China, and there is significant concern about the influence of microbes transported by Asian dust events on human health and downwind ecosystems. In this study, we collected Asian dust particles in Beijing, and analyzed the bacterial communities on these particles by culture-independent methods. Bacterial cells on Asian dust particles were visualized first by laser scanning microscopy, which demonstrated that Asian dust particles carry bacterial cells to Beijing. Bacterial abundance, as determined by quantitative polymerase chain reaction (PCR), was 10(8) to 10(9)cells/g, a value about 10 times higher than that in Asian dust source soils. Inter-seasonal variability of bacterial community structures among Asian dust samples, as compared by terminal restriction fragment length polymorphism (T-RFLP), was low during the Asian dust season. Several viable bacteria, including intestinal bacteria, were found in Asian dust samples by denaturing gradient gel electrophoresis (DGGE). Clone library analysis targeting 16S ribosomal RNA (rRNA) gene sequences demonstrated that bacterial phylogenetic diversity was high in the dust samples, and most of these were environmental bacteria distributed in soil and air. The dominant species in the clone library was Segetibacter aerophilus (Bacteroidetes), which was first isolated from an Asian dust sample collected in Korea. Our results also indicate the possibility of a change in the bacterial community structure during transportation and increases in desiccation-tolerant bacteria such as Firmicutes.


英文关键词Asian dust bacteria long-range transportation laser scanning microscopy 16S ribosomal RNA (rRNA) gene library phylogenetic analysis
类型Article
语种英语
国家Japan ; Peoples R China
收录类别SCI-E
WOS记录号WOS:000367359300008
WOS关键词FRAGMENT-LENGTH-POLYMORPHISM ; RIBOSOMAL-RNA ; DESERT-DUST ; MICROBIAL COMMUNITY ; SOIL DUST ; SP NOV. ; TRANSPORT ; ENVIRONMENTS ; DIVERSITY ; ARCHAEA
WOS类目Pharmacology & Pharmacy
WOS研究方向Pharmacology & Pharmacy
来源机构中国农业大学
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/191765
作者单位1.Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan;
2.Tottori Univ, Fac Agr, Tottori 6808553, Japan;
3.Osaka Univ, Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan;
4.Himeji Dokkyo Univ, Fac Pharmaceut Sci, Himeji, Hyogo 6708524, Japan;
5.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China
推荐引用方式
GB/T 7714
Yamaguchi, Nobuyasu,Baba, Takashi,Ichijo, Tomoaki,et al. Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season[J]. 中国农业大学,2016,39(1):68-77.
APA Yamaguchi, Nobuyasu.,Baba, Takashi.,Ichijo, Tomoaki.,Himezawa, Yuka.,Enoki, Kanami.,...&Nasu, Masao.(2016).Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season.BIOLOGICAL & PHARMACEUTICAL BULLETIN,39(1),68-77.
MLA Yamaguchi, Nobuyasu,et al."Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season".BIOLOGICAL & PHARMACEUTICAL BULLETIN 39.1(2016):68-77.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yamaguchi, Nobuyasu]的文章
[Baba, Takashi]的文章
[Ichijo, Tomoaki]的文章
百度学术
百度学术中相似的文章
[Yamaguchi, Nobuyasu]的文章
[Baba, Takashi]的文章
[Ichijo, Tomoaki]的文章
必应学术
必应学术中相似的文章
[Yamaguchi, Nobuyasu]的文章
[Baba, Takashi]的文章
[Ichijo, Tomoaki]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。