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This study was carried out to investigate the effects of long-term cultivation and landscape position on organic carbon content and soil aggregation. Sampling sites were determined based upon land use at the end of 50 years soil use and management, cultivated/annual wheat cropping and grazed pasture, and landscape position in Chaharmahal-va-Bakhtiary province, southwest Iran. Soil samples were collected from the 0-5 cm and 5-15 cm depths in two adjacent fields that have the same slope and aspect. The soil was silty clay at the summit and footslope positions, and was a silty clay loam at the backslope. Wet-sieving analysis and aggregate-size fractionation methods were used to separate the samples into three aggregate fractions (i.e., 2-4.75, 0.25-2, and 0.053-0.25 mm). The treatments were arranged in a factorial design. Land use significantly affected the water-stable aggregate fractions, so that the wet soil stability of the macroaggregates (i.e., 2-4.75 mm) was higher in the pasture, whereas it was greater for the meso-aggregates (i.e., 0.25-2 mm) in the cultivated soils. Cultivation decreased both the wet-aggregate stability and percent of macroaggregates whereas long-term pasture enhanced aggregation. Soil organic carbon (SOC) content within aggregates and primary particles was also significantly influenced by landscape position, land use, and the depth of sampling. The SOC content was higher in clay than those in silt and sand contents. The SOC content decreased as depth increased in all fractions. In general, the highest and lowest wet-stable aggregates were observed on the footslope and backslope positions, respectively.