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A Gram-negative, non-spore-forming, non-pigmented, strictly aerobic and non-motile short rod bacterium, designated NCCP-1258(T), was isolated from Cholistan desert soil, Bahawalpur, Pakistan. Growth of strain NCCP-1258(T) was observed at pH range 6.5-9.5 (optimum 7.5-8.5) and temperature range 20-45 A degrees C (optimum 40 A degrees C), and it tolerated 0-2 % NaCl (optimum 0.5 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain NCCP-1258(T) belongs to genus Microvirga and is most closely related to Microvirga lotononidis (98.0 %), Microvirga vignae (97.4 %), Microvirga lupini (97.2 %), Microvirga zambiensis (97.2 %) and Microvirga flocculans (97.1 %). Analysis of the concatenated sequences of four housekeeping gene loci (dnaK, gyrB, recA and rpoB) also confirmed the placement of strain NCCP-1258(T) within the genus Microvirga. DNA-DNA relatedness values of NCCP-1258(T) with above-mentioned type strains were less than 42 %. The DNA G+C content of strain NCCP-1258(T) was 64.3 mol%. Chemotaxonomic data (predominant menaquinone system was Q-10; major fatty acids were C-16:0, C-18:1 omega 7c and C-19:0 cyclo omega 8c; the polar lipid profile contained diphosphatidylglycerol, phosphatidylcholine, phosphatidyl dimethyl ethanolamine and phosphatidyl ethanolamine) also supported the affiliation of strain NCCP-1258(T) to the genus Microvirga. On the basis of physiological and biochemical characteristics, phylogenetic analyses and DNA-DNA relatedness, strain NCCP-1258(T) can be distinguished from the closely related taxa and thus represents a novel species of the genus Microvirga, for which the name Microvirga pakistanensis sp. nov. is proposed with the type strain NCCP-1258(T) (=CGMCC 1.15074(T) = KCTC 42496(T)).