Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1163/15685381-00003048 |
Development of 23 microsatellite loci for Boulenger’s agama (Agama boulengeri) with partial cross-amplification in other Agama species | |
Goncalves, Duarte Vasconcelos1,2,3; Pereira, Paulo1; Godinho, Raquel1,2,4; Lopes, Susana1; Velo-Anton, Guillermo1; Brito, Jose Carlos1,2 | |
通讯作者 | Goncalves, Duarte Vasconcelos |
来源期刊 | AMPHIBIA-REPTILIA
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ISSN | 0173-5373 |
EISSN | 1568-5381 |
出版年 | 2016 |
卷号 | 37期号:2页码:246-252 |
英文摘要 | Patterns of biodiversity and evolutionary processes controlling them are still poorly studied in desert biomes. Fine-scale markers could help answer some of the pressing research questions for desert biomes and Sahara in particular. Such markers are available for some large mammals and crocodiles, but not for small vertebrates. Here we present a battery of microsatellite loci developed for Agama boulengeri, a promising model to study evolutionary and demographic processes in the Sahara-Sahel. Loci were selected by sequencing enriched DNA libraries with 454 pyrosequencing. A total of 23 polymorphic loci were successfully amplified in four multiplex reactions. Cross-amplification of the microsatellite loci in A. agama and A. boueti was partially successful. These markers are a promising tool for assessing genetic diversity, gene-flow dynamics and demographic patterns in this group. Given the genus Agama is distributed throughout Africa, results presented here might also facilitate studies in other regions. |
英文关键词 | genetic diversity lizards Mauritania mountains population genetics Sahara-Sahel |
类型 | Article |
语种 | 英语 |
国家 | Portugal ; Spain ; South Africa |
收录类别 | SCI-E |
WOS记录号 | WOS:000375412200012 |
WOS关键词 | MAURITANIA WEST-AFRICA ; SAHARA DESERT ; BIOGEOGRAPHY ; SQUAMATA ; LIZARDS ; SYSTEMATICS ; GEKKONIDAE ; EVOLUTION ; SOFTWARE ; AGAMIDAE |
WOS类目 | Zoology |
WOS研究方向 | Zoology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/191232 |
作者单位 | 1.Univ Porto, Ctr Invest Biodiversidade & Recursos Genet, CIBIO InBIO, Campus Agr Vairao, P-4485661 Vairao, Portugal; 2.Univ Porto, Fac Ciencias, Dept Biol, Rua Campo Alegre, P-4169007 Porto, Portugal; 3.Univ Pompeu Fabra, CSIC, Inst Evolutionary Biol, Passeig Maritim Barceloneta 37-49, Barcelona 08003, Spain; 4.Univ Johannesburg, Fac Sci, Dept Zool, ZA-2006 Auckland Pk, South Africa |
推荐引用方式 GB/T 7714 | Goncalves, Duarte Vasconcelos,Pereira, Paulo,Godinho, Raquel,等. Development of 23 microsatellite loci for Boulenger’s agama (Agama boulengeri) with partial cross-amplification in other Agama species[J],2016,37(2):246-252. |
APA | Goncalves, Duarte Vasconcelos,Pereira, Paulo,Godinho, Raquel,Lopes, Susana,Velo-Anton, Guillermo,&Brito, Jose Carlos.(2016).Development of 23 microsatellite loci for Boulenger’s agama (Agama boulengeri) with partial cross-amplification in other Agama species.AMPHIBIA-REPTILIA,37(2),246-252. |
MLA | Goncalves, Duarte Vasconcelos,et al."Development of 23 microsatellite loci for Boulenger’s agama (Agama boulengeri) with partial cross-amplification in other Agama species".AMPHIBIA-REPTILIA 37.2(2016):246-252. |
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