Arid
DOI10.1163/15685381-00003048
Development of 23 microsatellite loci for Boulenger’s agama (Agama boulengeri) with partial cross-amplification in other Agama species
Goncalves, Duarte Vasconcelos1,2,3; Pereira, Paulo1; Godinho, Raquel1,2,4; Lopes, Susana1; Velo-Anton, Guillermo1; Brito, Jose Carlos1,2
通讯作者Goncalves, Duarte Vasconcelos
来源期刊AMPHIBIA-REPTILIA
ISSN0173-5373
EISSN1568-5381
出版年2016
卷号37期号:2页码:246-252
英文摘要

Patterns of biodiversity and evolutionary processes controlling them are still poorly studied in desert biomes. Fine-scale markers could help answer some of the pressing research questions for desert biomes and Sahara in particular. Such markers are available for some large mammals and crocodiles, but not for small vertebrates. Here we present a battery of microsatellite loci developed for Agama boulengeri, a promising model to study evolutionary and demographic processes in the Sahara-Sahel. Loci were selected by sequencing enriched DNA libraries with 454 pyrosequencing. A total of 23 polymorphic loci were successfully amplified in four multiplex reactions. Cross-amplification of the microsatellite loci in A. agama and A. boueti was partially successful. These markers are a promising tool for assessing genetic diversity, gene-flow dynamics and demographic patterns in this group. Given the genus Agama is distributed throughout Africa, results presented here might also facilitate studies in other regions.


英文关键词genetic diversity lizards Mauritania mountains population genetics Sahara-Sahel
类型Article
语种英语
国家Portugal ; Spain ; South Africa
收录类别SCI-E
WOS记录号WOS:000375412200012
WOS关键词MAURITANIA WEST-AFRICA ; SAHARA DESERT ; BIOGEOGRAPHY ; SQUAMATA ; LIZARDS ; SYSTEMATICS ; GEKKONIDAE ; EVOLUTION ; SOFTWARE ; AGAMIDAE
WOS类目Zoology
WOS研究方向Zoology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/191232
作者单位1.Univ Porto, Ctr Invest Biodiversidade & Recursos Genet, CIBIO InBIO, Campus Agr Vairao, P-4485661 Vairao, Portugal;
2.Univ Porto, Fac Ciencias, Dept Biol, Rua Campo Alegre, P-4169007 Porto, Portugal;
3.Univ Pompeu Fabra, CSIC, Inst Evolutionary Biol, Passeig Maritim Barceloneta 37-49, Barcelona 08003, Spain;
4.Univ Johannesburg, Fac Sci, Dept Zool, ZA-2006 Auckland Pk, South Africa
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GB/T 7714
Goncalves, Duarte Vasconcelos,Pereira, Paulo,Godinho, Raquel,等. Development of 23 microsatellite loci for Boulenger’s agama (Agama boulengeri) with partial cross-amplification in other Agama species[J],2016,37(2):246-252.
APA Goncalves, Duarte Vasconcelos,Pereira, Paulo,Godinho, Raquel,Lopes, Susana,Velo-Anton, Guillermo,&Brito, Jose Carlos.(2016).Development of 23 microsatellite loci for Boulenger’s agama (Agama boulengeri) with partial cross-amplification in other Agama species.AMPHIBIA-REPTILIA,37(2),246-252.
MLA Goncalves, Duarte Vasconcelos,et al."Development of 23 microsatellite loci for Boulenger’s agama (Agama boulengeri) with partial cross-amplification in other Agama species".AMPHIBIA-REPTILIA 37.2(2016):246-252.
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