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Stable isotopes of evapotranspiration (delta(ET)) provide us with useful information for tracing ecosystem processes in the study of biosphere-atmosphere interactions and hydrologic cycling. In this study, a numerical Iso-SPAC model with high frequency isotopic water vapor isotopic measurements was applied in an arid artificial oasis cropland ecosystem. The steady state assumption (SSA) and non-steady state (NSS) sub-models were tested for the isotopic composition of leaf water and transpiration flux. The modeled water/energy fluxes agreed well with eddy covariance observations, indicating that our model has the capacity to reproduce the observed water/energy flux behavior. Comparisons of modeled and estimated delta(ET) by the flux-gradient method using a cavity ring-down spectroscopy water vapor isotope analyzer as well as measured delta(L,b) indicate that delta(T) with SSA can reproduce better seasonal isotopic variations in ET flux and leaf water during the growing season. Uncertainties/errors for model output (delta(ET), delta(E), delta(T) and delta(L,b)) under both SSA and NSS were discussed quantitatively. With corn growth, the isoflux of transpiration acted to increase delta(ET) and decrease enrichment in delta(L,b), inversely, the delta(ET) decreased and isotope enrichment in delta(L,b) increased because of reduction of transpiration caused by frost occurred during 13th and 14th September. The results indicate that the Iso-SPAC model is a useful framework for simulating the dynamics of isotopic composition in the isotopic composition of plant transpiration and soil evaporation separately as well as for simultaneously integrating them for isotopic composition in the ET flux. (C) 2015 Elsevier B.V. All rights reserved.