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Many remotely sensed evapotranspiration (ET) calculations are based on algorithms that include an aerodynamic resistance-related parameter, which is significantly affected by heterogeneity and could cause the ET to be underestimated as the scale increases, and it is challenging to verify the ET estimation because of the lack of a bridge between satellite-based remote sensing and ground-based measurements. To address this issue, an approach using a "thermal infrared remote sensing + three-temperature model" is proposed and applied under multi-scale conditions. It is hypothesized that good consistency exists between multi-scale aggregations and ET estimates using the proposed method because it does not include aerodynamic resistance. The mothed was verified in the Zhangye Oasis during the first thematic experiment launched under the HiWATER project using extensive eddy correlation (EC) tower observations (a total of 16 ECtowers) and multi-scale thermal infrared datasets, including aerial photos (3 m, as a bridge between the ground and satellite measurements), ETM+ (60-m resolution), ASTER (90 m), and MODIS (1000 m). The results show that good agreement is obtained between the EC-measured" ET and that using remote sensing techniques under multi-scale conditions. The determination coefficients (R-2) between the measured and estimated latent heat flux (LE) values were 0.72, 0.81, 0.61, and 0.53 for the aerial photos, ETM+, ASTER and MODIS, respectively. The respective mean absolute errors (MAEs) were 63.64, 56.56, 59.32, and 80.34 Wm(-2), and the mean absolute percent errors (MAPEs) were 12.00,11.18, 10.78, and 14.60%, respectively. However, the estimated ET became slightly overestimated as the sensor resolution decreased. In addition, the variability of the heterogeneous landscape represented by the range of LE values and the standard deviation decreased gradually as the sensor resolution decreased. Aggregation (up-scaling) of ET from higher-resolution images could better represent the heterogeneity over land surfaces than the original (unaggregated) ET maps, and there is high consistency between multi-scale aggregation and estimation, with a minimum R-2 of 0.76 and a maximum root-mean-square error (RMSE) of 64.80 Wm(-2). The proposed methodology is concluded to be a feasible method for estimating ET under multi-scale conditions over heterogeneous landscapes. (C) 2016 Elsevier B.V. All rights reserved.